圣地红景天对糖尿病大鼠尿微白蛋白排泄率及肾脏TGF-β_1表达的影响  

Effect of Rhodiola Sacra on Micro-albumin Excretion Rate and Expression of TGF-β_1 in Kidney of Type 1 Diabetic Rats

作　　者：李彩萍[1] 张艳[1] 黄骁燕[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院综合科,武汉430030

出　　处：《中国中西医结合肾病杂志》2008年第10期871-874,共4页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：武汉市科技局攻关计划项目(No.200550030)

摘　　要：目的:观察圣地红景天对1型糖尿病大鼠尿微白蛋白排泄率及肾脏TGF-β1表达的影响并探索其机制。方法:SD雄性大鼠45只,单侧肾切除后随机分为:对照组15只(NC组),模型组30只,后者按55mg/kg体重单次腹腔注射1%链脲佐菌素(STZ)制作糖尿病模型。待造模成功后随机分为糖尿病模型组(DC组)、糖尿病治疗组(DR组)各15只。分别于第4、8、12周时检测各组血尿素氮、肌酐及尿肌酐、尿微白蛋白排泄率,同时测肾组织中丙二醛(MDA)含量,铜锌超氧化物歧化酶(Cu-Zn-SOD)及谷胱甘肽过氧化酶(GSH-Px)活性,留取肾脏组织分别作光镜、电镜检查及免疫组化检查TGF-β1的表达。结果:与NC组相比,DR组24h尿微白蛋白排泄率下降,肾组织中NDA含量下降,Cu-Zn-SOD及GSH-Px活性升高;与DC组相比,DR组尿蛋白排泄率减少,早期肾小球高滤过减轻,病理检查示系膜增生明显减轻,电镜示肾小球毛细血管基底膜增厚轻于DC组,免疫组化分析示DR组TGF-β1表达较DC组明显减少。结论:圣地红景天可以有效减轻糖尿病大鼠的肾脏损害,其对糖尿病肾脏的保护作用可能与其抗氧化应激及降低肾组织TGF-β1的表达有关。Objective： To investigate the effect of rhodiola .sacra on micro - albumin excretion rate and the expression of TGF- -β1 in kidney of type 1 diabetic rats. Methods：Fourty - five male SD rats with unilateral kidney removed were randomly divided into the control group（NC group, n = 15） and the diabetic model group（ n = 30）. Diabetic rats models that were established by single peritoneal injection of 1% STZ （55 mg/kg） were subdivided into the diabetic group （DC group, n - 15） and the diabetes treatment group（DR group, n - 15）. We detected the serum BUN, serum creatinine, urine creatinine,micro albumin excretion rate in each group at the 4th, 8th and 12th week respectively. The MDA level, Cu, Zn SOD and GSH - Px activity in kidney tissue were examined, pathology and ultrmierostructure and the expression of TGF --β1 were examined by microscope, electron miercxscope and immunohistoc hemistry respectively. Results：Compared with those in the NC group, micro- albumin excretion rate and the concentration of MDA in the kidney were significantly decreased in the DR group, while Cu, Zn - SOD and GSH - Px activity in kidney tissue were significantly increased in the DR group. Compared with those in the DC group, the micro albumin excretion rate was decreased;glomeruli high filtration and mesangial hyperplasia relieved and the degree of basal membrane thickening and the expression of TGF-β1 were significantly decreased in the DR group. Conclusion：Rhodiola sacra can effectively relieve the damage of kidney in the diabetic rats, its protective effect may be related to the decrease of oxidative stress and expression of TGF-β1.

关 键 词：圣地红景天 糖尿病 抗氧化酶 转化生长因子-Β1 

分 类 号：R285.5[医药卫生—中药学]