Characteristics of fat metabolism in skeletal muscle of rats after hypobaric hypoxic acclimation  

作　　者：Mao Sunzhong Gao Yuqi Chen Jian Liu Fuyi Gao Wenxiang Huang Jian Liao Weigong Cai Mingchun 

机构地区：[1]Department of Pathophysiology and High Altitude Physiology, College of High Altitude Military Medicine, Third Military Medical University, Chongqing 400038, China [2]Department of Basic Medicine, Wenzhou Medical College, Wenzhou 325035, China

出　　处：《Journal of Medical Colleges of PLA(China)》2008年第5期258-262,共5页中国人民解放军军医大学学报（英文版）

基　　金：the National Basic Research Program of China(2006CB504100);the National Natural Science Foundation of China (30393131, 30771043)

摘　　要：Objective：To investigate the characteristics of fat metabolism in rat skeletal muscle after hypobaric hypoxia acclimation. Methods： Sprague-Dawley rats were divided into 3 groups randomly： control group （H0）, hypoxic 5-day group （HS）, and hypoxic 15-day group （H15）. Animals of H5 and 15 groups were exposed to hypobaric hypoxia chamber simulating 5 000 m high altitude for 5 d or 15 d respectively, 23 h per day. H0 group stayed outside of chamber The level of fatty acid oxidation and uptake, and glucose oxidation were examined, and the level of non-esterified fatty acids （NEFA）, ATP and phosphocreatine （PCr） were also assayed in rat skeletal muscles. Results： The contents of ATP and PCr in H5 group were lower than those in H0 and H15 groups （P〈0.05）, while there was no significant difference between H0 and H15. Compared with H0, the blood NEFA level in all hypoxia groups was increased significantly （P〈0.05）. The muscle NEFA level in H15 group was greatly higher than that in H0 and H5 groups. The rates of fatty acid oxidation and uptake in H15 group were significantly higher than those in H0 and H5 groups （P〈0.05）, and the rate of glucose oxidation in all hypoxia groups was significantly decreased than that in H0 group （P〈0.05）. Conclusion： It is concluded that the enhanced fat oxidation may be one of the mechanisms in the maintenance of energy homeostasis after hypobaric hypoxic acclimation.Objective: To investigate the characteristics of fat metabolism in rat skeletal muscle after hypobaric hypoxia acclimation. Methods: Sprague-Dawley rats were divided into 3 groups randomly: control group (H0), hypoxic 5-day group (H5), and hypoxic 15-day group (H15). Animals of H5 and 15 groups were exposed to hypobaric hypoxia chamber simulating 5 000 m high altitude for 5 d or 15 d respectively, 23 h per day. H0 group stayed outside of chamber. The level of fatty acid oxidation and uptake, and glucose oxidation were examined, and the level of non-esterified fatty acids (NEFA), ATP and phosphocreatine (PCr) were also assayed in rat skeletal muscles. Results: The contents of ATP and PCr in H5 group were lower than those in H0 and H15 groups (P<0.05), while there was no significant difference between H0 and H15. Compared with H0, the blood NEFA level in all hypoxia groups was increased significantly (P<0.05). The muscle NEFA level in H15 group was greatly higher than that in H0 and H5 groups. The rates of fatty acid oxidation and uptake in H15 group were significantly higher than those in H0 and H5 groups (P<0.05), and the rate of glucose oxidation in all hypoxia groups was significantly decreased than that in H0 group (P<0.05). Conclusion: It is concluded that the enhanced fat oxidation may be one of the mechanisms in the maintenance of energy homeostasis after hypobaric hypoxic acclimation.

关 键 词：Energy metabolism Hypobaric hypoxia ACCLIMATION FAT 

分 类 号：R3[医药卫生—基础医学]