4种碘化钾-过氧化氢法髓过氧化物酶染色结果比较  

COMPARATIVE STUDY ON THE RESULTS OF FOUR DIFFERENT STAINING METHODS FOR MYELOPEROXIDASE USING POTASSIUM IODIDE AND HYDROGEN PEROXIDE

作　　者：陈万新[1] 朱红琳[1] 薛梅[1] 官阳[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院,武汉430022 [2]华中科技大学同济医学院病理学系,武汉430030

出　　处：《中国组织化学与细胞化学杂志》2008年第5期497-501,共5页Chinese Journal of Histochemistry and Cytochemistry

基　　金：华中科技大学同济医学院附属协和医院科研基金资助(2007)

摘　　要：目的为了探讨较理想的髓过氧化物酶(MPO)染色方法。方法采用我室新建立的碘化钾-吡啰红B法(KI-PyB法)及碘化钾-吡啰红G法(KI-PyG法),以及碘化钾-煌焦油蓝法(KI-BCB法)、碘化钾-Wright-Giemsa法(KI-WG法)四种MPO染色法,同时对80例骨髓涂片标本进行了MPO染色。结果KI-PyB法MPO阳性产物呈鲜红色颗粒状,细胞核为蓝绿色;KI-PyG法MPO阳性产物呈棕黑色颗粒状,细胞核为浅红色;KI-BCB法阳性产物为蓝黑色颗粒状,细胞核为紫红色;KI-WG法阳性产物为深红色至棕黑色颗粒状,细胞核为浅蓝色或浅紫红色。4种碘化钾法MPO染色平均阳性率分别为60.9%、62.1%、56.3%及61.3%,差异无统计学意义(P>0.05)。结论4种碘化钾法MPO染色中KI-PyG法的阳性率相对较高、且阳性反应易于判断,是较理想的MPO染色方法。Objective To find out an optimal myeloperoxidase （MPO） staining method. Methods Two staining methods designed by the authors Epotassium iodide - Pyronine B （KI-PyB） and potassium iodide - Pyronine G （KI-PyG）] and 2 other MPO staining methods, [Potassium iodide - Brilliant cresyl blue （KI-BCB） and potassium iodide - Wright-Giemsa （KI-WG）] were simultaneously applied to 80 bone marrow smear samples. Results The myelocytes stained with KI-PyB showed blue-green nuclei with bright red granules in the cytoplasm. KI-PyG method showed light red nuclei with brown-black granules in the cytoplasm. In KI-BCB-staining, blue-black granules were seen in the cytoplasm, while the nuclei were stained purplish red. In KI-WG staining, the positive products were dark red or brown-black, and the nuclei were light blue or purple. The positive rates of the different KI-MPO staining methods were 60.9%, 62.1%, 56.3% and 61.3%, which showed no significant difference （P〉0.05） . Conclusion With KI-PyG staining, the sensitivity is relatively higher, the positive reaction is more easily identified, and the dyeing re sults are more credible.

关 键 词：髓过氧化物酶 细胞化学 碘化钾 

分 类 号：R345.9[医药卫生—基础医学]