Cloning and Expression Analysis of a Prion Protein Encoding Gene in Guppy (Poecilia reticulata)  

作　　者：WU Suihan WEI Qiwei YANG GuanDin WANG Dengqiang ZOU Guiwei CHEN Daqing 

机构地区：[1]College of Marine Life Sciences, Ocean Universin, of China, Qingdao 266003, P R. China [2]Yangtze River Fisheries Research Institute, Chinese Academy of Fishery, Jingzhou 434000, P R. China

出　　处：《Journal of Ocean University of China》2008年第4期425-431,共7页中国海洋大学学报（英文版）

基　　金：the Key Laboratory of Freshwater Germplasm and Biotechnology of Chinese Ministry of Agriculture,Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences (LFB20070601);the Key Laboratory of Mariculture of Chinese Ministry of Education,Ocean University of China;National High Technology Research and Development Program of China (2007AA09Z427)

摘　　要：The full length cDNA of a prion protein (PrP) encoding gene of guppy (Poecilia reticulata) and the corresponding ge-nomic DNA were cloned. The cDNA was 2245 bp in length and contained an open reading frame (ORF) of 1545 bp encoding a pro-tein of 515 amino acids,which held all typical structural characteristics of the functional PrP. The cloned genomic DNA fragment corresponding to the cDNA was 3720 bp in length,consisting of 2 introns and 2 exons. The 5’ untranslated region of cDNA origi-nated from the 2 exons,while the ORF originated from the second exon. Although the gene was transcribed in diverse tissues in-cluding brain,eye,liver,intestine,muscle and tail,its transcript was most abundant in the brain. In addition,the transcription of the gene was enhanced by 5 salinity,implying that it was associated with the response of guppy to saline stress.The full length cDNA of a prion protein （PrP） encoding gene of guppy （Poecilia reticulata） and the corresponding genomic DNA were cloned. The cDNA was 2245 bp in length and contained an open reading frame （ORF） of 1545 bp encoding a protein of 515 amino acids, which held all typical structural characteristics of the functional PrP. The cloned genomic DNA fragment corresponding to the cDNA was 3720 bp in length, consisting of 2 introns and 2 exons. The 5＇ untranslated region of cDNA origi- nated from the 2 exons, while the ORF originated from the second exon. Although the gene was transcribed in diverse tissues in- cluding brain, eye, liver, intestine, muscle and tail. its transcript was most abundant in the brain. In addition, the transcription of the gene was enhanced by 5 salinity, implying that it was associated with the response of guppy to saline stress.

关 键 词：GUPPY Poecilia reticulata prion protein GENE expression analysis 

分 类 号：Q178.53[生物学—水生生物学]