格列美脲对2型糖尿病GK大鼠心肌缺血预适应的影响  

Effect of Glimepiride on isolated GK rat heart myocardial protection afforded by ischemic preconditioning

作　　者：张中琳[1] 吴国亭[1] 蒋国良[1] 

机构地区：[1]同济大学附属第十人民医院内分泌科,上海200072

出　　处：《同济大学学报（医学版）》2008年第5期19-22,共4页Journal of Tongji University(Medical Science)

基　　金：上海市科委科技攻关基金重点资助项目(04DZ19507)

摘　　要：目的研究磺脲类药物格列美脲对糖尿病GK大鼠离体心脏缺血预适应(ischemic preconditioning,IPC)保护作用的影响。方法采用GK大鼠离体心脏Langendorff灌流模型,动物随机分组:Wistar大鼠缺血预处理对照组(CON组);GK大鼠缺血预适应保护组(IPC组);缺血预适应+格列美脲组(10μmol/L)(IPC+Glm组)。观察格列美脲对心肌缺血预适应后不同时间点(1 min、3 min)冠脉流出液中AST、LDH、CK、CK-MB及冠脉流量,评价药物对心肌缺血预适应的影响。结果格列美脲未增加GK大鼠心肌缺血预处理后缺血/再灌注损伤的心肌冠脉流出液中AST、LDH、CK、CK-MB的含量(P>0.05),也未降低再灌注期间的冠脉流量(P>0.05)。结论格列美脲未影响缺血预适应对心肌的保护作用。Objective To study the effect of Glimepiride on isolated GK rat heart myocardial protection afforded by ischemic preconditioning（IPC）. Methods The isolated male Goto-Kakisaki Wistar rats＇ hearts were perfused by Langendorff system and randomly divided into 3 groups： ischemic preconditioning control group （CON group ）, ischemic preconditioning group （IPC group ） and ischemic preconditioning ＋ Glimepiride（ 10 μmol/L）（ IPC ＋ Glm group）. The effects of Glimepiride on coronary blood flow and the release of aspartate aminotransferase（AST）, lactate dehydrogenase （LDH）, creatine kinase （CK）, creatine kinase-MB （ CK-MB ） at different time （ 1 min ,3 min） after ischemia/reperfusion injury in GK rat Langendorff hearts were studied. Results Glimepiride did not increase the release of AST, LDH,CK,CK-MB （ P 〉 0.05） and the coronary blood flow （ P 〉 0.05 ）. Conclusion The results show that Glimepiride dose not abolish the myocardial protection afforded by ischemic preconditioning.

关 键 词：格列美脲 心肌缺血预适应 langendorff灌流 GK大鼠 

分 类 号：R587.1[医药卫生—内分泌]