表皮生长因子、胰岛素样生长因子-1体外诱导胎儿骨髓间充质干细胞分化  

Differentiation of fetal bone marrow mesenchymal stem cells induced by EGF and IGF-1 in vitro

作　　者：杨艳[1] 段征[1] 姜蓉[1] 汪维伟[1] 

机构地区：[1]重庆医科大学基础医学院组织学与胚胎学教研室,重庆400016

出　　处：《解剖学杂志》2008年第5期622-625,共4页Chinese Journal of Anatomy

基　　金：重庆医科大学创新基金(CX200307)

摘　　要：目的:研究表皮生长因子(EGF)和胰岛素样生长因子-1(IGF-1)体外诱导胎儿骨髓间充质干细胞(BMSCs)向上皮细胞的分化,及其对种植于羊膜上BMSCs的影响。方法:不同浓度EGF、IGF-1体外诱导第3代BMSCs培养7 d后,检测各组细胞角蛋白(CK)及上皮膜抗原(EMA)的阳性细胞率。检测去上皮羊膜有无EGF或IGF-1表达情况;观察生长因子对种植于羊膜上BMSCs的分化影响。结果:EGF 30 ng/ml或IGF-1 50 ng/ml组的CK及EMA阳性细胞率高于其余剂量组;两因子联合诱导组的CK及EMA阳性细胞率均高于各单因子组;30 ng/ml EGF和50 ng/ml IGF-1联合组的阳性细胞率最高;去上皮羊膜表达EGF,不表达IGF-1;BMSCs在羊膜上生长不受外源性生长因子影响。结论:EGF和IGF-1在体外均有诱导BMSCs向上皮细胞分化的作用;以30 ng/ml EGF和50 ng/ml IGF-1联合组的协同作用最强;种植于羊膜上的BMSCs可生长为复层上皮结构,羊膜中的EGF可能是其诱导作用的内源性因素。Objective： To study the differentiation of fetal bone marrow mesenchymal stem cells （BMSCs） induced by epidermal growth factor （EGF） and insulin-like growth factor-1 （IGF-1） in vitro and these effects on the BMSCs planted on amnion. Methods： The positive rate of cytokeratin （CK） and epithelial membrane antigen （EMA） of cells was detected in each group at 7 clays after P3-BMSCs being induced by different concentrations of EGF and IGF-1. Whether EGF and IGF1 was expressed in the amniotic membrane without epithelium was detected, and the differentiation of BMSCs planted on amnion with or without growth factors was observed. Results ： The positive cells of CK or EMA in the group of EGF 30 ng/ml or IGF-1 50 ng/ml was significantly higher than those of other concentration groups; The CK or EMA positive cells in the group induced with EGF and IGF-1 were significantly higher than those in the group induced with single factor; The CK positive cells in the group induced with 30 ng/ml EGF and 50 ng/ml IGF-1 was higher than those in other groups induced with two factors; EGF was expressed in the acellular amniotic membrane, but IGF-1 was not expressed. BMSCs formed stratified epithelium on the amnion with or without the exogenous growth factors. Conclusion： BMSCs could be induced to differentiate into epithelial cells by EGF and IGF-1 in vitro ; The optimal synergistic effect of EGF and IGF1 was in 30 ng/ml EGF and 50 ng/ml IGF-1 respectively. The BMSCs planted on the amnion could grow and form stratified epithelium structure. EGF in amnion may be one of the endogenetic reasons which induced BMSCs differentiate into epithelial cells.

关 键 词：生长因子 羊膜 骨髓间充质干细胞 分化 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]