福氏志贺菌4c亚型的分子生物学特征分析  

Analysis of the molecular biologic characterization of Shigella flexneri 4c isolates

作　　者：丁水军[1] 孙永祥[1] 汪皓秋[2] 傅丹青[1] 戴城钢[1] 

机构地区：[1]杭州市萧山区疾病预防控制中心卫生实验中心微生物部,311201 [2]杭州市疾病预防控制中心微生物检验科,310006

出　　处：《国际流行病学传染病学杂志》2008年第5期297-300,共4页International Journal of Epidemiology and Infectious Disease

摘　　要：目的了解杭州地区2003—2007年从爆发和散发细菌性痢疾患者中分离之福氏志贺菌4c亚型（F4c）菌株的分子生物学特征。方法对24株F4c菌株以生化反应和血清分型进行鉴定，利用PER法检测侵袭性质粒抗原H基因（ipaH）及脉冲场凝胶电泳（PFGE）进行分型分析。结果24株菌株全部为F4e，均具有ipaH毒力基因。用PFGE分型方法将24株菌株分成17种不同的带型，Dice系数为0．71～1．00。2005年、2006年、2003年三次爆发（分别简称为爆发1、2、3）中各自分离到的菌株基因型紧密相关。三次爆发中的部分菌株可能存在一定的相关性（Dice系数〉0．8）。散发菌株和爆发1、3菌株间的距离较远（Dice系数〈0．8），和爆发2菌株间可能存在一定的相关性（Dice系数〉0．8）。结论F4c菌株具有ipd／毒力基因，PFGE提示杭州地区近年F4c三起爆发菌株可能属共同的克隆群。Objective To analysis the molecular biological characteristics of Shigellaflexneri 4c isolates from patients of shigellosis outbreak and sporadic diarrhea in Hangzhou during 2003-2007. Method 24 S. flexner/4c isolates were identified by biochemical tests and serotyping. Among these isolates, invasive plasmid antigen H gene （ipaH） was examined by PCR, and molecular typing was performed by Pulse-field gel electrophoresis（PFGE）. Results All 24 isolates were serotyped as S. flexner/4c and were positive for ipaH gene. A total of 17 different PFGE patterns were identified among these 24 isolates with Dice coefficient varying from 0.71 to 1.00. On the basis of PFGE patterns, S. flexner/ 4c isolates from each of three shigellosis outbreaks were closely related. Some isolates from three outbreaks （outbreak in 2005,2006 and 2003 year which named as outbreak 1, 2,3 respectively） were possibly related （Dice coefficient 〉 0. 8）. The sporadic isolates were demonstrated to be distinct from the isolates from two outbreaks （outbreak 1,3） （Dice coeffieient 〈 0.8）. However, the sporadic isolates were possibly related to isolates from outbreak 2 （Dice coefficient 〉 0.8）. Conclusions All S. flexneri 4c in Hangzhou possess ipaH. The S. flexner/4c isolates from three recent outbreaks in Hangzhou may belong to the same clone.

关 键 词：志贺菌 4c亚型 缸洲 脉冲场凝胶电泳 分型 

分 类 号：R446.5[医药卫生—诊断学]