转化生长因子对成骨细胞增殖及其受体信号表达的影响  

Effects of biomaterial with transforming growth factor-beta (TGF-beta)1.on regulating proliferation activity and receptor signal gene expression in mouse osteoblast-like MC3T3-E1 cells

作　　者：王友君 姚航平[2] 林军[2] 

机构地区：[1]建德浙江省第一人民医院,311600 [2]浙江大学医学院附属一院口腔科

出　　处：《中国口腔种植学杂志》2008年第3期105-107,117,共4页Chinese Journal of Oral Implantology

摘　　要：目的:研究转化生长因子β1对小鼠成骨细胞增殖的调节作用,及其受体表达的变化,初步了解TGFβ1促成骨细胞增殖的分子机制。方法:以小鼠成骨细胞株MC3T3-E1为TGFβ1检测的细胞模型。10ng/mlTGFβ1作用MC3T3-E1细胞后,采用3H-TdR细胞增殖试验,检测成骨样细胞增殖改变,采用半定量反转录聚合酶链式反应(RT-PCR)检测TGFβI,TGFβⅡ受体mRNA的表达。结果:(1)在10ng/mL浓度时,TGFβ1对MC3T3-E1细胞有明显的增殖促进作用,使MC3T3-E1成骨样细胞在24小时内成倍增殖。含转化生长因子组TGFβI受体,TGFβⅡ受体的mRNA表达的水平高于空白对照组.(3)10ng/mLTGFβ1具有维持MC3T3-E1细胞活力的作用。(4)TGFβ1可能,通过MC3T3-E1细胞表面TGFβI受体,TGFβⅡ受体途径表达,传递信号。Objective： To evaluate the effects of TGFβ1 on regulating the levels of proliferation activity and the mRNA transcriptions of TGF-betal receptor in mouse osteoblast-like cells. Methods： Mouse osteoblastic cell line MC3T3-E1 was selected as the effective cell of TGF-betal. After the cells were treated. [^3H]-Thymidine copration assays was used for examining the cell proliferation. RT-PCR employed for determining the levels of TGF-betal receptor mRNAs. Results： After the MC3T3-E1 cells were treated with TGF-betal at the dosages of 1 0 ng/mlfor 24h, [^3H]-Thymidine copration assays increased markedly （P 〈 0.05 or P 〈 0.01）. the levels of TGF-betal receptors mP, NA increased significantly （P 〈 0.01）. Conclusions： TGF-betal at the dosages of 10ng/mL showed the effects on promoting proliferation, which probably leaded to the maintenance of high TGF-betal receptors levels. TGF- beta1 receptor I gene expression at the level of transcription was up regulated in the MC3T3-E1 cell treated with dosage of TGF-betal （10 ng/ml） and TGF-betal receptor Ⅱ mRNA was also, which might be one of the mechanisms for the maintenance of high TGF-betal receptor levels.TGF-betal （10 ng/ml） induce immediate early gene expression and growth in MC3T3-E1 osteoblasts primarily through pathway of TGF-betal receptors.

关 键 词：转化生长因子 成骨样细胞 转化生长因子受体 增殖活性 

分 类 号：R68[医药卫生—骨科学]