海岛棉抗黄萎病性状分子标记的研究及QTL的定位  

Molecular Mark of Resistance to Verticillium wilt of G. barbadence and Mapping of QTL

作　　者：昝伟[1] 高峰[1] 刘海峰[2] 李国英[1] 宋武[2] 罗城[2] 李晖[1] 

机构地区：[1]新疆生产建设兵团绿洲生态农业重点实验室／石河子大学,新疆石河子832003 [2]新疆天彩科技股份有限公司,乌鲁木齐830000

出　　处：《新疆农业科学》2008年第5期805-808,共4页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区科技攻关重点项目“棉花重要经济性状分子标记育种技术研究和新型材料选育”子课题“棉花黄萎病分子标记研究(200311101)”

摘　　要：以高抗黄萎病海岛棉品种新海15号和高感陆地棉品种新彩棉1号为材料,通过对其F2分离群体和F2∶3家系进行QTL研究,构建了一个包括11个连锁群、标记间平均间距为8.6 cM、全长547 cM的海陆种间分子标记遗传连锁图。应用WinQTLCartV2.5软件,利用复合区间作图法(CIM)原理对相对病情指数进行了全基因组扫描和基因定位分析,检测到2个跟抗黄萎病性状紧密连锁的QTLs,都位于连锁群1上,并分别解释F2∶3群体的表型变异为46.74%和48.69%,初步认为海岛棉新海15号抗黄萎病性状由两个主效QTLs共同控制。研究所获得的这两个QTLs是主效的,且与抗黄萎病性状紧密连锁,为抗黄萎病育种奠定基础,对加速棉花育种进程具有一定意义。A genetic linkage map was constructed using F2 and F2：3 individuals , which was developed by a cross G. barbadense cv Xinhai15 C, osssypium hirsutum cv Xincaimianl. Eleven linkage groups were constructed spanning 547 cM with an average distance of 8.6 cM between markers, QTL analysis was performed with WinQTLCartV2.5. Two QTLs were detected on one linkage group, and explained 46.74% and 48.69% of the phenotypic variance respectively which might lay the foundation of resistance to V. wilt and could promote cotton breeding effectively.

关 键 词：棉花 抗黄萎病 QIL 分子标记 

分 类 号：S641.1[农业科学—蔬菜学] S511[农业科学—园艺学]