阿霉素纳米粒对P-gp介导的膀胱肿瘤多药耐药细胞株BIU-87/ADM多药耐药性的逆转作用  

Reversal effect of adriamycin-loaded nanoparticles on P-gp mediated bladder tumor multidrug-resisitance in cell line BIU-87/ADM

作　　者：王磊[1] 柯红[2] 

机构地区：[1]三峡大学医学院,湖北宜昌443002 [2]葛洲坝中心医院,湖北宜昌443002

出　　处：《中国医院药学杂志》2008年第20期1743-1746,共4页Chinese Journal of Hospital Pharmacy

摘　　要：目的:研究阿霉素纳米粒对P-gp介导的膀胱肿瘤多药耐药的逆转作用。方法:采用甲基噻唑基四唑(MTT)法测定药物的体外杀伤作用,应用流式细胞术测定细胞内药物浓度。结果:阿霉素纳米粒和阿霉素对BIU-87的细胞毒作用相似,BIU-87/ADM对阿霉素纳米粒较阿霉素敏感3.50倍,细胞内阿霉素浓度显著增加可能是关键因素。结论:阿霉素纳米粒通过增加耐药细胞内阿霉素浓度有效逆转多药耐药。OBJECTIVE To investigate reversal effect on bladder tumor multidrug resistance（MDR） by adriamycin-loaded nanoparticles. METHODS The reversal effect of the drugs on multidrug-resistance was determined by MTT method. Intracellular accumulation of ADR was studied by flow cytometry. RESULTS The cytotoxicity of adriamycin-loaded nanopartides and free adriamycin was identical to BIU-87 cells, but the chemosensitivity of BIU-87/ADR cells to adriamycin-loaded nanoparticles was increased to 3. 50 times compared with that of BIU-87/ADR cells to free adriamycin. The increased sensitivity was due to a remarkable increasing intracellular accumulation of adriamycin. CONCLUSION Adriamycin-loaded nanoparticles reverse MDR effectively by increasing intracellular accumulation of adriarnycin in BIU-87/ADR cells.

关 键 词：阿霉素 聚氰基丙烯酸异丁酯 纳米粒 多药耐药 逆转 

分 类 号：R96[医药卫生—药理学]