甘草总黄酮微丸溶出度的考察  

Determination of the dissolution of glycyrrhiza total flavones pellets by ultraviolet spectrophotometry

作　　者：陈丽华[1,2] 徐德生[1] 冯怡[1] 陈莉[1] 

机构地区：[1]上海中医药大学,上海201203 [2]江西中医学院,江西南昌330004

出　　处：《中国医院药学杂志》2008年第20期1758-1761,共4页Chinese Journal of Hospital Pharmacy

基　　金：上海市重点学科建设项目资助(编号:T0301);上海市科委项目资助(编号:04DZ19841);高等学校博士学科点专项科研基金资助项目(编号:20060268009)

摘　　要：目的:建立甘草总黄酮微丸溶出度的测定方法。方法:依据中国药典2005年版二部附录XC,采用桨法测定,以1.0%十二烷基硫酸钠的pH6.8磷酸盐缓冲溶液为溶出介质,转速为100r.min-1,紫外分光光度法检测。结果:以甘草苷为对照品计算累积溶出率,45min时均大于75%,在4.07～24.42μg的范围内,浓度与其吸收度呈良好线性关系,平均回收率为100.61%,RSD为0.95%。结论:经过溶出条件的系统筛选后所建立的方法稳定,结果可靠。OBJECTIVE To establish a method for determining the dissolution of glycyrrhiza total flavones pellets by Ultraviolet spectrophotometry. METHODS The determination was performed at. the detection wavelength 05 335nm, solvent： 1.0%SDS-pH 6. 8 phosphate buffer, paddle rate： 100 r·min^-1, temperature： 37 ℃. RESULTS Compared with liquiritin as a standard, the cumulative dissolution percentage exceeded 75% in 45 minutes. Glycyrrhiza total flavones concentration presented a good linear range of 4. 07-24. 42μg; the average recovery was 100. 61% with RSD of 0. 95%. CONCLUSION The method is simple, accurate and reliable for determining the dissolution of glycyrrhiza total flavones.

关 键 词：甘草总黄酮微丸 溶出度 紫外分光光度法 甘草苷 

分 类 号：R285.5[医药卫生—中药学]