星半通膈散诱导人食管癌Eca-109细胞凋亡及其机制研究  

Effect of Xingban Tongge Powder in Inducing Apoptosis of Human Esophageal Cancer Cell Eca-109 and Its Mechanism

作　　者：刘华东[1] 王明艳[1] 张民庆[1] 黄仕文[1] 梁枫[2] 

机构地区：[1]南京中医药大学基础医学院,江苏南京210046 [2]安徽中医药高等专科学校,安徽芜湖241000

出　　处：《南京中医药大学学报》2008年第5期332-334,366,共4页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏省中医药局课题(H05168)

摘　　要：目的研究星半通膈散药液诱导人食管癌Eca-109细胞凋亡及其作用机制。方法采用MTT法测定细胞生长抑制率,通过流式细胞仪观察凋亡细胞及检测p53、bcl-2基因表达。结果星半通膈散不同剂量药液均可抑制人食管癌Eca-109细胞生长,诱导细胞凋亡,降低p53(突变型)、bcl-2蛋白表达率。结论星半通膈散能诱导人食管癌Eca-109细胞凋亡,而抑制p53基因突变、下调bcl-2基因表达可能是其重要机制。OBJETIVE To investigate into the effect of Xingban Tongge Powder in inducing apoptosis of human esophageal cancer cell Eca-109 and its mechanism. METHOD MTT method was used to determine cell inhibiting rate, and flow cytometry was used to observe cell apoptosis and examine p53 and bcl-2 gene expression. RESULT At different dosages, Xingban Tongge Powder could inhibit the growth of human esophageal cancer cell Eca-109, induce cell apoptosis and reduce p53 and bcl-2 gene expression. CONCLUSION Xingban Tongge Powder can induce apoptosis of human esophageal cancer cell Eca-109,the mechanism of which is probably related to the inhibition of p53 mutation and decrease of bcl-2 gene expression.

关 键 词：星半通膈散 人食管癌Eea-109细胞 凋亡 P53 BEL-2 实验研究 

分 类 号：R273.51[医药卫生—中西医结合]