正交试验设计优选紫草的醇提工艺  

Optimization on Alcohol Extraction Technology of Radix Arnebiae by Orthogonal Design

作　　者：吴学渊[1] 刘萍[2] 

机构地区：[1]成都中医药大学药学院,成都市610075 [2]解放军总医院中药房,北京市100853

出　　处：《中国医院用药评价与分析》2008年第10期750-752,共3页Evaluation and Analysis of Drug-use in Hospitals of China

基　　金：解放军总医院新药研发基金(NDR0706)

摘　　要：目的:优选紫草中左旋紫草素等萘醌类成分的最佳提取工艺。方法:采用L9(34)正交试验法,以左旋紫草素量和干浸膏得率为评价指标,考察乙醇用量、提取温度和提取时间3个因素,每个因素选取3个水平进行试验。结果:提取温度对紫草有效成分的提取有显著影响,最佳提取工艺为:加入12倍和10倍量95%乙醇,在65℃条件下温浸提取两次,每次1h。结论:优选得到的工艺稳定、合理可行。OBJECTIVE： To optimize the extraction technology of naphthoquinone such as L- shikonin in Radix Arnebiae. METHODS： An L9 （34） orthogonal experiment was performed to investigate the 3 factors （three levels for each factors were selected）, i.e. the amount of ethanol, temperature and time of extraction with the quantity of L - shikonin and extracta sicca yield as indexes for evaluation. RESULTS： The temperature of extraction had significant impact on the extraction of the effective components in Radix Arnebiae. The best extraction technology was as follows： adding 12 - fold and 8 - fold ethanol solution （at a concentration of 95% ） for digeratur extraction twice at 65℃（1.0 h each time ）. CONCLUSION： The optimized technology for the extraction of L - shikonin from Radix Arnebiae was stable, reasonable and feasible.

关 键 词：紫草 左旋紫草素 正交试验 提取工艺 

分 类 号：R932[医药卫生—生药学]