红花桑寄生总黄酮提取物诱导淋巴瘤细胞株CA46凋亡及其分子机制研究  

A Total Flavonoids Extract of Scurrula parasitic L.Parasitized on Nernium indicum Mill.Inducing Human Burkitt's Lymphoma Cell Line CA46 Apoptosis and the Related Molecular Mechanism

作　　者：肖义军[1] 刘奋[1] 陈元仲[2] 陈建华[1,2] 陈炳华[1] 范延丽[1] 

机构地区：[1]福建师范大学生命科学学院,福州350108 [2]福建医科大学附属协和医院福建省血液病研究所,福州350001

出　　处：《天然产物研究与开发》2008年第5期797-802,共6页Natural Product Research and Development

基　　金：福建省教育厅基金项目(JA05201)

摘　　要：研究了一种寄主为夹竹桃的红花桑寄生总黄酮提取物(Nispex)对人Burkitt淋巴瘤细胞株CA46的抗肿瘤作用,探讨了其抗肿瘤作用的分子机制。应用MTT法研究Nispex对CA46细胞增殖的抑制效果,细胞集落培养法观察Nispex对CA46中增殖细胞群的影响。采用AO/EB荧光染色、TUNEL分析、DNA凝胶电泳分析以及AnnexinV流式细胞术检测细胞凋亡,Western blot检测Nispex对CA46细胞中NF-κBp65、Bcl-2、Bax、Caspase-3和PARP等蛋白表达的影响。结果表明,Nispex显著抑制CA46细胞增殖和诱导CA46细胞凋亡,作用48h的IC50值为1.72μg/mL,细胞凋亡率与药物浓度正相关。Nispex能有效上调CA46细胞Bax、Caspase-3蛋白表达,下调NF-κBp65、Bcl-2、PARP蛋白表达。Nispex诱导CA46细胞凋亡可能是通过对NF-κB信号通路的抑制来实现的。The anticancer activity of a total flavonoids extract of Scurrula parasitic L. parasitized on Nernium indicum Mill. （Nispex） on human Burkitt＇ s lymphoma cell line CA46 and its molecular mechanism was studied here. The growth inhibition activity of CA46 cell was detected by MTT assay and colony formation assay. The cell apoptosis induced by Nispex was detected by AO/EB fluorescence staining,TUNEL analysis, DNA fragmentation assay and AnnexinV-FITC/ PI flow cytometry assay, and the expression of protein related with cell apoptosis was detected by Western blot assay. The results showed that Nispex remarkably inhibited the proliferation of CA46 cell in a dose-dependent and time-dependent manner,with an IC50 value of 1.72 μmL. Concomitantly,Nispex induced CA46 cell apoptosis also. The expressions of Bax,Caspase-3 protein were enhanced and NF-κBp65, Bcl-2, PARP protein were decreased after Nispex treatment in CA46 cell. These results indicated that Nispex could efficiently inhibit cancer cell＇ s growth and induce its apoptosis ; the inhibition of NF-κB signal pathway may be involved in the process of CA46 cell apoptosis induced by Nispex.

关 键 词：红花桑寄生 黄酮 CA46 细胞凋亡 NF-ΚB 

分 类 号：R282.71[医药卫生—中药学] R733.7[医药卫生—中医学]