志苓胶囊对小细胞肺癌细胞株NCI-H446细胞凋亡及h-TERT、CD44表达的影响  

Effects of Zhiling capsule on apoptosis and expressions of hTERT,CD44 in small cell lung cancer cell line NCI-H446

作　　者：陈英玉[1] 潘云苓[2] 郑志宏[1] 潘明继[2] 胡建达[1] 刘庭波[1] 

机构地区：[1]福建医科大学附属协和医院,福建省血液病研究所,福建福州350001 [2]福州市第一医院肿瘤科,福建福州350001

出　　处：《中国药理学通报》2008年第10期1382-1386,共5页Chinese Pharmacological Bulletin

基　　金：福建省科技重点资助项目(No2004Y023)

摘　　要：目的研究志苓胶囊(抗癌复方Ⅱ号)对人小细胞肺癌细胞株NCI-H446诱导凋亡作用机制以及对细胞黏附分子表达的影响。方法将志苓胶囊按其不同的中西药成份配制成相应的中药组、西药组和复方组,与NCI-H446共培养后,倒置显微镜观察、Hochest33258荧光染色法检测凋亡细胞,RT-PCR、Western blot、流式细胞仪分别检测bcl-2、bax、hTERT基因mRNA和相应蛋白表达水平变化以及黏附分子CD44的表达变化。结果NCI-H446经不同的药物组处理后,倒置显微镜、Hochest33258荧光染色可观察到细胞凋亡形态学改变,bcl-2基因mRNA和蛋白表达水平均有不同程度下降,bax基因表达水平则有不同程度增强,复方组改变最明显,各药物组与对照组比较差异具有显著性(P<0.01)。中药组和西药组hTERT基因和蛋白表达水平未见明显改变,复方组表达水平下调,与对照组比较差异具有显著性(P<0.01)。各药物组CD44表达水平降低,其中复方组降低水平最明显(P<0.01)。结论志苓胶囊可能通过下调NCI-H446细胞bcl-2、hTERT基因和CD44分子表达,增强bax基因表达,从而诱导NCI-H446细胞凋亡,抑制细胞黏附、转移。Abstract：Aim To explore the mechanisms of the apoptosis induction and the effects of adhesion suppression of Zhiling capsule （ZLJN） in small cell lung cancer cell line NCI-H446. Methods According to the different components of ZLJN, NCI-H446 cells were treated with traditional Chinese medicine, western medicine and ZLJN composite groups. Apoptotic cells were tested by light microscopy, Hochest33258 staining method. The mRNA and protein expressions of bcl-2, bax and hTERT were analyzed by RT-PCR and Western blot respectively. The expressions of CD44 were detected by flow cytometry. Results After NCI-H446 cells were treated with different drug groups, The morphological changes of apoptotic cells were found by light microscopy and Hoehest33258 staining method. The mRNA and protein expressions of bcl-2 were down-regulated while the expressions of bax were upregulated compared to the control groups （ P 〈 0. 01 ）. The expressions of hTERT in traditional Chinese medicine and western medicine groups show no changes, but the expression in composite group was significantly lowered compared to the control group （ P 〈 0. 01 ）. The expressions of CD44 were also decreased in each group. All the changes were the most significant in composite group （P 〈 0.01 ）. Conclusion ZLJN could efficiently induce apoptosis in NCI-H446 cells and suppress the cell adhesion and metastasis, which maybe related to suppression of the expressions of bcl-2, hTERT and CD44 and activation of the expression of bax.

关 键 词：小细胞肺癌细胞株NCI-H446 志苓胶囊 凋亡 HTERT CD44 

分 类 号：R273.42[医药卫生—中西医结合] R289.1[医药卫生—中医肿瘤科]