右旋柠烯对大鼠结直肠癌E-钙黏蛋白和β-连环素表达的影响  

Impact of D-limonene on the expressions of E-cadherin and β-catenin in colorectal cancer in rats

作　　者：刘艳翠[1] 李玉兰[1] 徐贞刚[1] 刘岩[1] 杨慧科[1] 

机构地区：[1]哈尔滨医科大学基础医学院人体解剖学教研室,黑龙江哈尔滨150081

出　　处：《解剖学研究》2008年第5期333-335,343,共4页Anatomy Research

基　　金：黑龙江省科技厅自然科学基金(D200625);黑龙江省卫生厅科学技术课题(2006-471);黑龙江省教育厅科学技术课题(10553050)

摘　　要：目的观察D-limonene(右旋柠烯)对大鼠结直肠癌的生长和E-cad(E-钙黏蛋白,E-cadherin)、β-cat(β-连环素,β-catenin)表达的影响,从分子学角度探讨其抗肿瘤的机制。方法皮下注射致癌剂二甲基肼(dimethylhydrazine,DMH)构建大鼠原发肠癌模型,中期后给予D-limonene干预,免疫组化染色观察癌组织E-cad、β-cat的表达情况。结果E-cad和β-cat在两组均有异常表达,治疗组异常表达率低;β-cat对照组异位表达增加。结论D-limonene降低了E-cad、β-cat异常表达率,可能抑制了肿瘤的发展和转移。Objective To observe the impact of D-limonene on the growth of colorectal cancer in rats and on the expressions of E-cadherin （E-cad）, β-catenin （β-cat） and to explore its anti-tumor mechanism from the perspective of molecular. Methods Subcutaneous injection of carcinogenic agents of DMH were used to construct the primary model of colorectal cancer, D- limonene interference was performed in the medium-term. The expression of E-cad, β-cat was observed by immunohistoehemical staining. Results Abnormal expression of E-cad and β-cat was detected in both treatment and control groups. The abnormal expression rate was lower in treatment group and the ectopic expression rate of β-cat in the control group was higher. Conclusion D-limonene reduced the E-cad, β-cat abnormal expression rate and may inhibit tumor development and transfer.

关 键 词：右旋柠烯 E-钙黏蛋白 Β-连环素 结直肠癌 

分 类 号：R96[医药卫生—药理学] R735.3[医药卫生—药学]