胃癌死亡相关蛋白激酶基因甲基化对其信使核糖核酸和蛋白表达的影响  

The promoter methylation of death-associated protein kinase influences expressions of its mRNA and protein in tissue of gastric cancer

作　　者：叶梅[1] 夏冰[1] 李冬青[2] 周峰[1] 郭秋莎[1] 

机构地区：[1]武汉大学中南医院消化科,430071 [2]武汉大学医学院免疫学系

出　　处：《中华消化杂志》2008年第10期678-681,共4页Chinese Journal of Digestion

基　　金：湖北省自然科学基金资助项目（2007ABA261）;湖北省科技攻关项目（2004AA301C91）

摘　　要：目的研究胃癌组织死亡相关蛋白激酶（DAPK）基因启动子区甲基化对原发性胃癌组织中DAPK mRNA及蛋白表达的影响。方法采用逆转录（RT）-PCR法检测62例原发性胃癌及癌旁组织DAPK mRNA表达，甲基化特异性PCR（MSP）法检测DAPK启动子区CpG岛甲基化状态，对其中34例胃癌组织甲基化阳性者的DAPK蛋白表达进行Western印迹法检测。结果胃癌组织中DAPK mRNA和蛋白表达水平明显低于癌旁组织，分别为0．2863±0．2027比0．5736±0．1968、0．2616±0．0913比0．6529±0．1808，差异均有统计学意义（P值均〈0．01）。DAPK在胃癌组织和癌旁组织中的甲基化频率分别为54．8％和17．7％，差异有统计学意义（P〈0．01）。在胃癌组织中，甲基化组DAPK mRNA表达明显低于非甲基化组（0．1399±0．0835比0．4640±0．1569，P〈0．01）。DAPK基因甲基化与胃癌TNM分期显著相关（P=0．04）。结论原发性胃癌组织DAPK mRNA和蛋白表达缺失或低下与其启动子甲基化程度增高显著相关。Objective To investigate the regulation effect of promoter methylation of deathassociated protein kinase （DAPK） on mRNA and protein expression of DAPK in tissue of primary gastric cancer （GC）. Methods The cancerous and noncancerous samples from 62 patients with GC were determined by RT-PCR for mRNA expression of DAPK. The DAPK promoter methylation was detected by methylation-specific PCR. The protein expression of DAPK in 34 patients with methylation was determined by Western blot. Results mRNA and protein expressions of DAPK in cancerous tissues were reduced significantly compared to s tissues （0. 2863±0. 2027 vs 0. 5736±0. 1968,0. 2616±0. 0913 vs 0. 6529 ±0. 1808, P〈0.01）. Met hylation frequency of DAPK in cancerous tissues was higher than that in noncancerous tissues （54.8% vs 17.7%, P〈0.01）. Furthermore, DAPK mRNA expression was decreased in methylation group compared to unmethylation group （0. 1399 ±0. 0835 vs 0. 4640 ± 0. 1569, P〈0. 01）. Moreover, a significant correlation was demonstrated between the TNM stage and DAPK promoter methylation （P = 0.04）. Conclusion Expression of DAPK is down-regulated in cancerous tissues at mRNA and protein levels. Low expression of DAPK is associated with hypermethylation of the promoter of DAPK gene.

关 键 词：胃肿瘤 死亡相关蛋白激酶 甲基化 

分 类 号：R686[医药卫生—骨科学]