核因子-κB和激活蛋白-1在恶唑酮诱导的小鼠实验性肠炎中的表达  

The expressions of nuclear factor -κB and activator protein-1 in oxazoione induced colitis in mice

作　　者：陆允敏[1] 陈维雄[1] 陈金联[1] 朱金水[1] 陈尼维[1] 

机构地区：[1]上海市第六人民医院消化科,200233

出　　处：《中华消化杂志》2008年第10期682-685,共4页Chinese Journal of Digestion

基　　金：上海市自然科学基金资助项目（05ZR14085）

摘　　要：目的研究恶唑酮诱导的小鼠实验性肠炎中核因子（NF）-κB和激活蛋白（AP）-1基因表达量的变化，以探索其在发病机制中的意义。方法7～8周龄的健康昆明小鼠24只，体重25～30g，均分为正常组和模型组。模型组小鼠采用3％恶唑酮皮肤致敏5d后，以0．5％恶唑酮（溶解于50％乙醇中）0．15ml一次性灌肠造模方式建立实验性结肠炎模型。3d后处死所有小鼠，分别提取外周血单个核细胞（PBMC）、脾脏单个核细胞（SMC）和肠黏膜固有层单个核细胞（LPMC）。经细胞mRNA抽提、逆转录eDNA后进行荧光定量PCR（Taqman探针法）检测NF-κB、AP-1的表达量。并进行结肠炎的组织学评分。结果模型组NF-κB在SMC、LPMC和PBMC中的表达量均比正常组明显增高（分别为5．62±0．78比3．16±0．59、5．46±0．38比3．18±0．58、5．65±0．56比3．36±0．59，P〈0．01），AP-1亦是如此（分别为5．61±0．54比3．22±0．50、5．50±0．69比3．19±0．40、5．67±0．44比3．27±0．41，P〈0．01）。结论NF-κB、AP-1的活化可能与结肠炎的发病机制有关。Objective To investigate the expression changes of nuclear factor（NF）-κB and activator protein （AP）-1 in oxazolone induced colitis in mice and their mechanisms. Methods Twenty-four mice were randomly divided into normal group and model group with 12 each. Experimental colitis was induced with skin sensitization of 3% oxazolone for 5 days, then rectal administration of 0. 15 ml of 0. 5% oxazolone solution in mice. All mice were sacrificed on day 3. Peripheral blood mononuelear cells （PBMC）, spleen mononuclear cells （SMC） and lamina propria mononuelear cells （LPMC） were isolated from the colon tissues. Expression of NF-κB and AP-1 in SMC, LPMC and PBMC were determined by fluorescence quantitative polymerase chain reaction （PCR）. The colitis was evaluated histologically. Results The expressions of NF-κB and AP-1 in SMC, LPMC, PBMC of model group were significantly higher than those in normal group（NF-κB ： 5.62±0.78 vs. 3. 16±0.59,5.46±0.38 vs. 3. 18±0.58, 5.65±0.56 vs. 3.36±0.59, P〈0.01; AP-1： 5.61±0.54 vs. 3.22±0.50, 5.50±0.69 vs. 3.19± 0.40,5.67 ± 0. 44 vs. 3. 27± 0. 41, P〈 0. 01）. Conclusion The activation of NF-κB and AP-1 are involved in the mechanisms of ulcerative colitis.

关 键 词：溃疡性结肠炎 核因子-ΚB 激活蛋白-1 恶唑酮 

分 类 号：R686[医药卫生—骨科学]