玫瑰基因组AFLP反应体系的建立和优化  

DNA Template Preparation and Technology System Building for AFLP Analysis of Rosa Rugosa.

作　　者：李玉舒[1] 张启翔[2] 

机构地区：[1]北京农业职业学院,北京102442 [2]北京林业大学,北京100083

出　　处：《北京农业职业学院学报》2008年第5期28-33,共6页Journal of Beijing Vocational College of Agriculture

基　　金："十五"国家科技攻关资助项目(2004BA525B11)的部分成果

摘　　要：以玫瑰(Rosa rugosa)叶片为材料,利用改良的CTAB法,提取到了高质量的玫瑰叶片DNA,并建立了37个玫瑰品种和5个蔷薇品种的AFLP银染反应体系,首次在玫瑰DNA提取、酶切连接、预扩增、选择性扩增等实验过程取得了成功,得到了清晰的玫瑰品种的AFLP指纹图谱,为玫瑰基因库的建立、优良品种选育以及亲缘关系的系列研究奠定了理论基础。Extraction of DNA with phenol - cholorofore method from Rosa rugosa leaves was dealt with and AFLP finger printing in 37 R. rugosa cultures and 5 Rosa davurica were established. Clear and high resolution DNA finger prints were obtained, which mena success in DNA extraction, restriction - ligasereaction, pre-amplification and staining techniques. A foundation for the establishment of gene pool, selection and breeding of eminent cultivars and geneic relaionshpi in R. rugosa was laid.

关 键 词：玫瑰 DNA CTAB法 AFLP 指纹图谱 

分 类 号：S68[农业科学—观赏园艺]