盐胁迫对水稻幼苗类囊体膜脂肪酸组分的影响  

Effects of Salt Stress on Fatty Acid Composition of Thylakoid Membrane of Two Rice Cultivars

作　　者：王仁雷[1] 华春[2] 周峰[2] 周泉澄[2] 周斌伟[2] 

机构地区：[1]江苏食品职业技术学院,江苏淮安223003 [2]南京晓庄学院生命科学系,江苏南京211171

出　　处：《安徽农业科学》2008年第28期12108-12111,共4页Journal of Anhui Agricultural Sciences

基　　金：江苏省高校青蓝工程学术带头人项目

摘　　要：[目的]探讨盐胁迫下水稻幼苗类囊体膜脂肪酸组分变化。[方法]6叶1心的水稻幼苗在不同浓度NaCl胁迫不同时间后用于提取类囊体膜脂肪酸,并用1890气相色谱仪分析脂肪酸组分。[结果]随着NaCl胁迫浓度增加和时间的延长,2个供试材料Pokkali和Peta水稻类囊体膜14∶0,18∶0,16∶1(3t),18∶1脂肪酸组分含量在前4 d变化不大,之后略为上升;Pokkali和Peta的16∶0、饱和脂肪酸含量(LS-FA)前4 d变化也不大,之后Pokkali的明显上升,而Peta的呈下降趋势;Pokkali和Peta的18∶3组分和不饱和脂肪酸指数(IUFA)一直下降,100 mmol/L较200 mmol/L NaCl处理下降幅度小,且在相同的条件下,Pokkali较Peta的下降幅度小;而Pokkali和Peta的饱和度水平上升,且Peta上升幅度较Pokkali小。[结论]在不饱和脂肪酸指数方面,Pokkali较Peta耐盐。[ Objective ] The aim of the study is to understand the changes of fatty acid composition of flee thylakoid membrane under salt stress. [ Method] Under salt stress of different coneentrations of NaCl, rice seedlings of Pokkali and Peta with six leaves and one central leaf were used as experimental materials to extract the fatty acid from their thylakoid membranes, and gas chromatograph（1890） was used to analyze fatty acid composition. [Result ] Fatty acid component 14：0, 18：0, 16：1 （3t）, 18：1 in both the two experimental materials showed little variations in the first four days of salt stress, whereafter they increased slightly; while the fatty acid component 16：0 and level of saturation of fatty acid （ LSFA ） showed the similar variation trend in the first four days of treatment compared to thcse of the fatty acid compononts mentioned above, whereafter they rose in Pokkali and presented an opposite variation trend in Peta; fatty acid component 18：3 and level of unsaturation of fatty acid （LUFA） reduced all the time under stress condition, and the reducing amplitude in 100 mmol/L NaCl treatment group was smaller than that of 100 mmol/L NaCl treatment group, and in Pokkah was smaller than that in Peta under specific conditions. Meanwhile level of saturation of fatty acid in both experimental materials increased, and the rising amplitude in Peta was smaller than that of Pokkali. [Conclusion] With regard to LUFA, Pokkali is endowed with mere salt tolerance than Peta.

关 键 词：盐胁迫 水稻 脂肪酸 

分 类 号：S311[农业科学—作物栽培与耕作技术]