大鼠嗅成鞘细胞培养不同分离方法对纯度的影响  

Effect of different harvesting methods on the purity of olfactory ensheathing cells from rat olfactory bulbs

作　　者：程梦华[1] 贾强[1] 董璇[2] 姚柏春[2] 

机构地区：[1]郧阳医学院麻醉学系,2004级湖北省442000 [2]郧阳医学院人体解剖学教研室,湖北省442000

出　　处：《医学动物防制》2008年第11期805-807,共3页Journal of Medical Pest Control

基　　金：郧阳医学院基础医学院No.2006xsky01;No.2005ky02;湖北省教育厅B200624005

摘　　要：目的通过对多种分离方法的比较获得较为理想的嗅成鞘细胞的分离措施。方法应用3种不同分离方法各取12份样本,对同一条件下培养10d的细胞纯度进行p75NGFR免疫细胞化学测定及统计分析;结果(1)3种不同分离方法取材平均活细胞率:传统分离法(组)为84.52%,直接挤压法(组)为97.33%,机械过滤法(组)为83.97%,直接挤压(组)法分散后的OECs活细胞数量最多(P<0.05);(2)3种分离方法取材、培养所获得的OECs纯度:传统分离法(组)为67.58%,直接挤压法(组)为91.23%,机械过滤法(组)为63.51%;直接挤压法(组)培养细胞纯度最高(P<0.05)。结论嗅成鞘细胞最佳分离措施为直接挤压法,应用直接挤压法可收获大量嗅成鞘细胞,对OECs进一步的基础研究以及将来的临床应用奠定了基础。Objective To develop the better methods of drawing the materials from rat olfactory bulbs and puring OECs. Methods Twelve samples were obtained respectively by three different harvesting methods. Cellular purity of culturod OECs after culturing 10 days under identical condition were identified with nerve growth factor receptor p75 （NGFRp75） immunohistocbemistry. Results （1） Average alive cells rate with crushing directly is 97.33%, This data was obviously superior to traditional separation （84. 52% ） and mechanical filtering （ 83.97% ） . （2） Crushing directly would gain higher cells purity （91.23%） . It was obviously superior to traditional separation （67.58%） and mechanical filtering （63. 51% ） . Conclusion The best method of drawing the materials from the rat olfactory bulbs was crushing directly. A large number of pured OECs will provide convenience for further basal study and clinical application in future.

关 键 词：嗅球 嗅成鞘细胞 培养 

分 类 号：R424.31[医药卫生—临床医学]