β-七叶皂苷钠对大鼠肠缺血再灌注损伤的保护作用  

The protection effect of β-aescin for ischemia reperfusion injury of intestine in rats

作　　者：张信来[1] 刘陶迪[1] 罗力[1] 李得溪[1] 候明星[1] 

机构地区：[1]内蒙古医学院第一附属医院肿瘤外科,内蒙古呼和浩特100059

出　　处：《中国普通外科杂志》2008年第10期993-997,共5页China Journal of General Surgery

摘　　要：目的研究β-七叶皂苷钠(β-aescin,β-A)对大鼠缺血再灌注(I/R)后肠黏膜的保护作用,并探讨其作用机制。方法88只健康雄性Wistar大鼠,随机分为2组:I/R对照组和I/Rβ-A预处理组,每组按I/R不同时点(0,1,2h),观察组织病理损害,Haglund评分,肠含水率,肠毛细血管通透性,肠黏膜上皮细胞调亡率,血清TNF-α,IL-6含量变化及生存率。结果与对照组比较,预处理组的肠黏膜组织病理损害,肠含水率,毛细血管通透性,血清TNF-α,IL-6水平(P<0.01)及肠黏膜细胞凋亡率均明显降低(P<0.05),而且生存率明显提高(P<0.05)。结论β-A对肠I/R后肠黏膜具有保护作用;其机制可能与抑制炎症细胞因子,降低I/R后毛细血管通透性及降低肠黏膜细胞调亡率有关。Objective To investigate the protection effect of β-aescin on rat intestine ischemia /reperfusion （I/R） injury. Methods Eighty-eight male wistar rats were randomly divided into two groups, then they were distinguished by different time point after occurrence of rcperfusion at 0 h, 1 h, and 2 h. We observed the changes of the serum tumor necrosis factor-α, interleukine-6, the apoptosis of intestine mucosal epithelial cell, intestinal microvascular permeability, and the survival rate. Results The group with β-aescin pretreatment was observed to have reduction of intestinal histopathological damage, and significant decrease of Haglund scores, intestinal microvascular permeability, intestinal water content and the level of TNF-α and IL-6 （P〈0.01） , and inhibition of intestinal mucosa cell apoptosis （P〈0.05） and mortality rate during 3 6 hours （P〈0.01） compared to controlled group with I/R after reperfusion. Conclusions The β-aescin pretreatment could protect the intestinal mucosa from I/R injury, and it might be related to suppression of proinflammatory factors, reduction of intestinal mucosa cell apoptosis and decreased intestinal microvascular permeability.

关 键 词：缺血再灌注损伤 肠 Β-七叶皂甙钠 肿瘤坏死因子Α 白细胞介素6 毛细血管通透性 细胞调亡 大鼠 

分 类 号：R656.7[医药卫生—外科学]