大鼠膀胱ICC样细胞与逼尿肌神经调控关系的形态学研究  

Morphological study on the role of ICC-like cells in detrusor neuromodulation of rat urinary bladder

作　　者：方强[1] 杨景 潘进洪[1] 李为兵[1] 沈文浩[1] 李龙坤[1] 王永权[1] 封建立[1] 丁砺蠡[1] 宋波[1] 

机构地区：[1]第三军医大学第一附属医院全军泌尿外科研究所,重庆400038 [2]第三军医大学第一附属医院病理研究所,重庆400038

出　　处：《中华外科杂志》2008年第20期1542-1545,共4页Chinese Journal of Surgery

基　　金：国家自然科学基金资助项目（30700270,30672093）

摘　　要：目的从形态学上探讨膀胱ICC样细胞在逼尿肌神经调控中的作用。方法采用透射电镜观察大鼠膀胱内ICC样细胞、神经和逼尿肌细胞之间的超微结构关系。通过c—kit免疫荧光染色对大鼠膀胱ICC样细胞进行鉴定，并通过c-kit与PGP9．5免疫荧光双标观察ICC样细胞与神经的结构关系。结果透射电镜显示，ICC样细胞与逼尿肌细胞紧密相邻处可见典型缝隙连接。在局部区域可见ICe样细胞的突起与神经末梢联系紧密。c-kit免疫荧光染色显示，大鼠膀胱内ICC样细胞主要位于黏膜下层、肌束边缘以及肌细胞间。c-kit与PGP9．5免疫荧光双标显示ICC样细胞与神经末梢在结构上关系紧密。结论从形态学上看，膀胱内ICC样细胞具备参与逼尿肌神经调控的结构基础，进一步从功能学上予以证实将有助于全面阐明逼尿肌神经调控理论。Objective To investigate the role of ICC-like cells in bladder neuromodulation in rat urinary bladder. Methods 14 SD rats and 1 guinea pig were sacrificed in this study. The ultra structural relationships among interstitial cells, nerves and detrusor smooth muscle cells （DSMCs） of urinary bladder were investigated by transmission electron microscopy（TEM）, c-kit immunofluorescence was used to identify ICC-like cells in SD rat urinary bladder and the structural relationship between ICC-like cells and nerve terminals was studied by immunofluorescence （double-label）. Results Gap junction between ICC-like cells and DSMCs was confirmed by TEM. ICC-like cells were very close apposition with nerve terminals under TEM. ICC-like cells were identified to exist in suburothlium layer, along the longititude of smooth muscle bundles and among detrusor smooth, muscle in SD rat urinary bladder by c-kit immunofluorescence. Double- labeled tissue with c-kit and PGP9.5 antibodies also showed that ICC-like cells were very close apposition with nerve terminals in SD rat bladder. Conclusions Morphological study indicated that ICC-like cells in rat urinary bladder may play an important role in detrusor neuromodulation. Further study on function will be helpful for elucidating the mechanism of bladder neuromodulation clearly.

关 键 词：膀胱 ICC样细胞 逼尿肌细胞 神经调控 

分 类 号：R686[医药卫生—骨科学]