炎性介质脂多糖、γ干扰素活化星形胶质细胞效应  

Activation of cultured mouse astrocytes by LPS and IFN-γ

作　　者：熊加祥[1,2] 白云[1] 宋敏[1] 许雪青[1] 王艳艳[1] 杨晓亚[1] 

机构地区：[1]第三军医大学基础医学部医学遗传学教研室,重庆400038 [2]第三军医大学基础医学部生理学教研室,重庆400038

出　　处：《第三军医大学学报》2008年第22期2065-2068,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金海外青年学者合作研究基金(30228018);第三军医大学校管课题(200503)~~

摘　　要：目的研究炎性介质脂多糖(LPS)、γ干扰素(IFN-γ)刺激离体星形胶质细胞的效应。方法LPS、IFN-γ刺激离体培养纯化的小鼠星形胶质细胞,ELISA检测细胞分泌TNF-α,Griess法测定NO浓度,激光共聚焦扫描显微镜(CLSM)检测细胞内游离钙浓度([Ca2+]i)。结果LPS(500ng/ml)、IFN-γ(100U/ml)刺激星形胶质细胞24h后,NO和TNF-α的分泌显著升高,联合应用有协同作用;延长IFN-γ作用时间,分泌量升高。CLSM检测LPS、IFN-γ刺激24h后的星形胶质细胞[Ca2+]i的荧光值,显示荧光相对值比正常细胞明显升高;LPS、IFN-γ急性刺激正常培养细胞发现[Ca2+]i振荡上升;而作用于IFN-γ刺激24h后的细胞,[Ca2+]i上升明显减缓。结论LPS、IFN-γ可以活化星形胶质细胞,表现为[Ca2+]i升高,TNF-α和NO分泌上调。Objective To investigate the effects of inflammatory chemokine LPS and/or IFN-γ on astrocytes. Methods After LPS and IFN-γ applied to the purified cultured mouse astroeytes, TNF-α and nitric oxide （NO） secreted from astrocytes and intracellular calcium concentration （ [ Ca2＋] i） were detected by ELISA, Griess and confocal laser scanning microscopy, respectively. Results Treatment with LPS （500 ng/ml） or IFN-γ （100 U/ml） for 24 h significantly increased the concentration of TNF-α and NO. Coadministration of LPS and IFN-γ showed a synergistic effect on the astrocytes. By using Ca2＋ imaging, we found that [ Ca2＋]i signal was significantly increased after LPS and/or IFN-γ treatment for 24 h. Also, acute treatment with LPS or IFN-γ could evoke Ca2＋ oscillations. In contrast, after application of these stimuli over 24 h, the Ca2＋ signal was increased more slowly. Conclusion Application of LPS and/or IFN-γ evoked TNF-α and NO secretion and [ Ca2＋ ]i elevation in cultured astroeytes, suggesting that astrocytes could be activated by LPS and IFN-γ.

关 键 词：脂多糖 Γ干扰素 星形胶质细胞 活化 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学] R338.1[医药卫生—基础医学]