链球菌蛋白对人肝癌Bel-7402细胞及人正常肝细胞生长的影响  

Effects of Streptococcal Protein on the Human Hepatocellular Carcinoma Bel-7402 Cells and Human Normal Liver Cells

作　　者：李彦芹[1,2] 王艳[1,3] 曹宁[1] 平国玲[1] 李卫红[4] 张力平[1] 

机构地区：[1]首都医科大学基础医学院病原生物学系 [2]解放军总医院呼吸内科 [3]北京积水潭医院检验科 [4]首都医科大学基础医学院细胞生物学系

出　　处：《首都医科大学学报》2008年第5期605-609,共5页Journal of Capital Medical University

基　　金：北京市教育委员会科技发展计划(KM200410025008)资助项目~~

摘　　要：目的观察链球菌蛋白对人肝癌Bel-7402细胞及人Chang氏正常肝细胞生长增生的影响。方法常规培养链球菌,提取细菌全蛋白并通过阴离子交换层析获得蛋白分离组分SpⅠ和SpⅡ。不同浓度链球菌蛋白(10、50、100mg/L)作用于人肝癌Bel-7402细胞及人Chang氏正常肝细胞24～72h后,采用MTT法检测两种细胞的增生活性。倒置显微镜下观察50mg/L链球菌全蛋白和SpⅡ对两种细胞的形态学变化。结果MTT结果显示,链球菌全蛋白及SpⅡ能显著抑制Bel-7402细胞的生长,与蛋白剂量和作用时间呈负相关关系;SpⅠ对Bel-7402细胞未显示出明显增生抑制作用。链球菌全蛋白及SpⅡ对人Chang氏正常肝细胞显示出不同程度的刺激生长作用。倒置显微镜下观察,链球菌全蛋白及SpⅡ作用后的Bel-7402细胞,细胞稀疏、脱落、排列紊乱,失去正常形态,而人Chang氏细胞未发现明显的形态学变化。结论链球菌全蛋白及SpⅡ能抑制人肝癌Bel-7402细胞的增生,并且能在一定程度上促进人Chang氏正常肝细胞增生。Objective To investigate the effects of streptococcal protein on the growth of human hepatocellular carcinoma Bel- 7402 cells and healthy human Chang＇ s liver cells, Methods The Streptococci（ NO：32080 strain） were cultured conventionally. The whole streptococcal protein was extracted according to related references. The streptococcal protein components Sp Ⅰ and Sp Ⅱ were separated by using DEAE-Sepharose Fast Flow anion exchange chromatography. Bel-7402 cells and Chang＇ s liver cells were treated with different concentrations（ 10, 50, 100 mg/L） of the whole bacterial protein, Sp Ⅰ or Sp Ⅱ for 24 -72 h. MTT assay was used to evaluate the effects of the proteins on the proliferation of two kinds of cells. The morphological changes of the two cells were observed by 50 mg/L whole streptococcal protein and Sp Ⅱ under the phase-contrast microscope. Results The growth of Bel-7402 cells was inhibited significantly in a time and dose dependent manner by the whole protein and Sp Ⅱ , while there was no significant growth inhibition of Sp Ⅰ. The whole streptococcal protein and Sp Ⅱ could promote the proliferation of Chang＇ s liver cells after the cells treated by the proteins. After treating with the whole streptococcal protein and Sp Ⅱ , Bel-7402 cells lost the normal morphology and changed sparsely and mussily, while Chang＇ s liver ceils changed little. Conclusion The whole protein and Sp Ⅱ of Streptococcus （ NO：32080 strain） can inhibit Bel- 7402 cell proliferation and stimulate the growth of human normal liver cells in vitro.

关 键 词：链球菌 人肝癌Bel-7402细胞 人Chang氏正常肝细胞 

分 类 号：R378.12[医药卫生—病原生物学]