天冬氨酸异羟肟酸对大鼠血压以及血管重塑的影响  

Effect of hydroxamate on blood pressure and vascular remodeling in rats

作　　者：赵霞[1] 金红芳[1] 杜淑旭[1] 陈跃[2] 唐朝枢[3] 杜军保[1,3] 

机构地区：[1]北京大学第一医院儿科,北京100034 [2]北京大学医药卫生分析中心,北京100083 [3]教育部分子心血管病学重点实验室

出　　处：《基础医学与临床》2008年第10期1025-1029,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金重点项目(30630031);国家杰出青年科学基金(30425010);国家重点基础研究发展规划(2006CB503807)

摘　　要：目的观察二氧化硫(SO2)生成酶抑制剂天冬氨酸异羟肟酸(HDX)对大鼠血压以及血管结构的影响。方法4周龄雄性自发性高血压大鼠(SHR)及WKY大鼠随机分为对照组及HDX组(每组各8只,共32只)。5周后检测血压、胸主动脉与血浆SO2水平、GOT活性、增值指数(PI)和p-ERK蛋白表达,胸主动脉显微形态结构。结果HDX干预后,WKY大鼠血压、中膜厚度与内径之比、PCNA、p-ERK蛋白表达均明显升高,而血浆及胸主动脉SO2水平及GOT活性明显降低(P<0.05或P<0.01);SHR胸主动脉与血浆SO2水平、GOT活性明显低于WKY组(P<0.05或P<0.01)。结论内源性SO2是维持机体血压及血管结构正常的重要因素之一。Objective To investigate the effect of hydroxamate （ HDX, inhibitor of endogenous sulfur dioxide production） on blood pressure and vascular structure in rat. Methods 4-week-old SHRs （ spontaneously hypertensive rats） and WKY （Wistar-Kyoto） rats were divided into control and HDX-treated group （respectively, n = 8）. Five weeks later, the blood pressure, SO2 content and GOT activity in serum and aorta, morphometric parameters, proliferative index （PI） and the expression of p-ERK were determined. Results Compared with WKY rats, the blood pressure, ratio of wall thickness to lumen radius, PI and the expression of p-ERK in aorta were increased, while SO2 content and GOT activity in serum and aorta were decreased in WKY ＋ HDX groups （P 〈 0. 05 or P 〈 0. 01 ）. S02 content and GOT activity in serum and aorta of SHR were lower than those of WKY rat （ P 〈 0. 05 or P 〈0. 01 ）. Conclusion Endogenous sulfur dioxide may play a pivotal role in maintaining normal pressure and vascular structure.

关 键 词：二氧化硫 高血压 SHR 

分 类 号：R96[医药卫生—药理学]