纳米转基因技术可用于大鼠心梗后血管再生  

Nanometer transgenic technology and angiogenesis after myocardial infarction in rats

作　　者：唐红梅[1] 廉瑞青[2] 张晓东[3] 

机构地区：[1]中国医学科学院北京协和医学院基础医学研究所细胞生物学系,北京100005 [2]中国医学科学院北京协和医学院基础医学研究所人体解剖与组织胚胎学系,北京100005 [3]清华大学医学院,北京100084

出　　处：《基础医学与临床》2008年第10期1035-1039,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(30170483)

摘　　要：目的探讨包载VEGF165质粒的聚乳酸-乙醇酸(PLGA)纳米粒子对大鼠心肌梗死后血管再生治疗的可行性。方法制备大鼠心梗模型36只,实验组24只、对照组12只,术后1周在梗死区和与正常心肌交界处注射pVEGF165-PLGA纳米粒子和pVEGF165。应用RT-PCR和免疫组化检测血管内皮生长因子在不同的时间点(3、7、11、14和21 d)的表达;组织切片观察梗死区血管形成特点及其密度;以及纳米粒子对人体的毒副作用。结果与对照组相比,实验组VEGF可在心肌组织持续稳定表达;梗死区血管内皮细胞增生活跃,再生血管数量增加。注射48 h后心肌细胞核内可见纳米粒子,8周后组织活检未见血管瘤。结论PLGA纳米粒子有效介导pVEGF165转染心肌,并通过心肌细胞表达VEGF165,促进缺血区心肌组织的血管再生。Objective To investigate the feasibility of the gene therapy on angiogenesis after myocardial infarction in rats. Methods Thirty-six male SD rats, after the ligation of left anterior descending coronary artery, were divided into 2 groups as experimental and control one. Expressions of VEGF were measured by RT-PCR and Immunohistochemistry （IHC）. Angiogenesis and capillary density were evaluated by HE stain, and qualitative and quantitative analysis were carried out. The adverse effects were tested after injection ofpVEGF165-PLGA nanoparticle. Results Compared with control groups, ischemic myocardial cells persistently and stably expressed VEGF in experimental group; Vascular endothelial cells actively proliferated, and the effect of angiogenesis was significant; 48 hours later, nanoparticles were observed in myocardial cells. Conclusion Injection of with pVEGF165-PLGA nanoparticle, it can stimulate effective host-derived angiogenesis, which results in the prevention of impaired cardiac muscle after myocardial infarction. It may be an effect way to treat MI.

关 键 词：心肌梗塞 纳米粒子 血管再生 转基因技术 

分 类 号：R542.22[医药卫生—心血管疾病]