不明原因无精症及少精症的AZF微缺失的分析  

Correlation among unexplained oligospermatism,azoospermatism and micro-deletion in AZF gene

作　　者：张香改 黄海燕 赵邦荣 董秀娟 刘杰 于俊荣 谭凤钦 

机构地区：[1]河北省计划生育科学技术研究院,河北石家庄050071

出　　处：《基础医学与临床》2008年第10期1057-1059,共3页Basic and Clinical Medicine

摘　　要：目的探讨男性不育患者中无精症、少精症与Y染色体基因(无精子因子,AZF)微缺失的关系,建立一个完整的适合中国人的AZF微缺失筛查的临床基因诊断方法。方法对62例无精症、少精症患者及20例正常男性采用多重聚合酶链反应法进行AZF区基因微缺失检测。结果44例无精症患者中存在6例缺失,占13.64%,18例少精症患者中存在4例缺失,占22.22%,缺失以AZFc区为主,20例正常男性对照AZF无缺失。结论Y染色体AZF微缺失是不明原因无精症、少精症的主要原因之一。Objective To investigate the association among oligospermatism, azoospermatism in male sterility patients and micro-deletion in AZF gene of Y chromosome and to establish a integrate clinical diagnostic method for screening micro-deletion in AZF gene in Chinese. Methods PCR method was used to detect micro-deletion in AZF gene in 62 oligospermatism and azoospermatism patients and 20 normal male controls. Results 13.64% （6/44） of oligospermatism patients and 22.22% （4/18） of azoospermatism patients presented micro-deletion. However, micro-deletion was not found in 20 normal male controls. Furthermore, micro-deletion occur mostly in AZFc region. Conclusion Micro-deletion of AZF gene from Y chromosome is one of the major risks for oligospermatism and azoospermatism.

关 键 词：无精症 少精症 AZF基因 多重聚合酶链反应 基因微缺失 

分 类 号：R698.2[医药卫生—泌尿科学]