化学与酶促相结合合成人生长激素基因  

Synthesis of Human Growth Hormone Gene by the Combination of Chemical and Enzymatic Method

作　　者：赵向阳[1] 高朝辉[1] 金璐[1] 王曦[1] 孟庆繁[1] 滕利荣[1] 

机构地区：[1]吉林大学生命科学学院,长春130012

出　　处：《高等学校化学学报》2008年第10期1995-1998,共4页Chemical Journal of Chinese Universities

摘　　要：根据人生长激素(hGH)的氨基酸序列和大肠杆菌密码子的偏好性,全面优化hGH的密码子,添加表达调控元件后合成序列的全长为1040 bp,采用化学方法拆分成30条单链寡核苷酸.采用改进的两步法拼接成全基因,得到2个全序列正确的基因.DA-PCR和OE-PCR拼接产物经T7核酸内切酶Ⅰ处理,合成基因中的碱基错误率降低了93.93%.According to the amino acid sequence and codon preference of E. coli, the human growth hormone （hGH） gene was optimized to avoid rare codons. The total length of the synthesized gene was 1040 bp because a promoter and a signal peptide sequence were added into it. Thirty oligonucleotides were designed and synthesized. The whole DNA sequence was synthesized by the improved two-step method. The sequencing result show that two complete correct gene were synthesised. The error rate of synthesized gene treated by T7 endonuclease Ⅰ reduced 93.93% in comparison with the standard two-step synthesis method.

关 键 词：人生长激素 基因合成 两步法 

分 类 号：Q516[生物学—生物化学] O629.8[理学—有机化学]