5种植物精油对小菜蛾幼虫的触杀作用及两种酶活性的影响  

Effect of five plant essential oils on contact toxicity and two enzymes of diamondback moth(Plutella xylostella)

作　　者：孙红霞[1,2] 占志雄[2] 吴珍泉[1] 黄玉清[2] 

机构地区：[1]福建农林大学植物保护学院,福建福州350002 [2]福建省农业科学院植物保护研究所,福建福州350013

出　　处：《福建农业学报》2008年第3期274-276,共3页Fujian Journal of Agricultural Sciences

基　　金：福建省科技计划项目(2005I004);福建省青年科技人才创新项目(2004J010)

摘　　要：采用点滴法测定花椒(Zanthoxylumbungeanum Maxim)、艾蒿(Artemisia argyi Levl. et Vant)、马尾松针(Pinus massoniana Lamb)、侧柏[Platycladus orientalis(Linn.)Franco]、黄蒿(Artemisia annua L.)5种植物精油对室内小菜蛾2龄幼虫的触杀活性,结果表明:随着精油剂量升高,幼虫的死亡率也相应升高,其中黄蒿精油处理对小菜蛾幼虫的毒性最高,160μL.mL-1时校正死亡率达51.73%;5种精油处理对小菜蛾AChE和CarE的活性均有抑制作用,其中,松针精油、花椒精油分别对AChE、CarE的抑制作用最强,抑制率达23.94%、37.07%。The toxicity of the essential oils of five plants, i. e. , Zanthoxylum bungeanum Maxim, Artemisia argyi Levi. et Vant, Pinus massoniana Lamb, Platycladus orientalis （L.） Franco, Artemisia annua L. were observed in vitro on the 2nd instar larvae of diamondback moth, Plutella xylostella in lab. The result showed a correlated mortality rate of the larvae with increasing concentration of the essential oils. A. annua oil was found to be most effective in killing the larvae, with a mortality rate of 51.73% at the dosage of 160 μL · mL^-1. The result of biochemical analysis indicated that the essential oils inhibited acetylcholinesterase （ACHE） and carboxylesterase （CarE） activities. Ache and Care activities were inhibited by P. massoniana and Z. bungeanum essential oils the greatest, at the rates of 23.94%0 and 37.07%, respectively.

关 键 词：植物精油 小菜蛾 触杀毒力 酶 

分 类 号：S436.341.24[农业科学—农业昆虫与害虫防治] S481.1[农业科学—植物保护]