松材线虫鉴定方法的研究与比较  

Comparative Studies on Identification Methods of Bursaphelenchu xylophilus

作　　者：张奇[1] 孙丹[2] 张楚菁[3] 李海燕[4] 马洪周[5] 熊惠龙[4] 王金成[3] 马以贵 杨文博[5] 白钢[1,5] 

机构地区：[1]南开大学药学院,天津300071 [2]南开大学学报编辑部,天津300071 [3]天津市出入境检验检疫局,天津300457 [4]国家林业局森林病虫害防治总站,辽宁沈阳110034 [5]南开大学生命科学学院,天津300071

出　　处：《南开大学学报（自然科学版）》2008年第5期43-49,共7页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家"十五"重点科技攻关子项目

摘　　要：通过形态学观察,同工酶分析,ITS-RFLP以及RAPD分析等方法分别对来自于不同国家和地区的40个线虫群体进行鉴定,并对四种分析方法进行比较.形态学观察可以初步区分松材线虫和拟松材线虫;通过谷草转氨酶的同工酶分析,可以准确地区分上述两种线虫;基于分子生物学的ITS-RFLP分析除了可以准确地鉴定两种线虫外,还可以对拟松材线虫的地域特征作出判断,划分为亚洲型和欧洲型;而RAPD分析不仅能准确地鉴定两种线虫,还可以描述各种松材线虫和拟松材线虫种内以及种间的遗传进化关系.The 40 isolates of pine wood nematodes were collected from various countries and areas, and identified by morphological characteristics, iso-enzyme analysis, ITS-RFLP and RAPD method respectively. Comparing with described above method, morphological characteristics could distinguish Bursaphelenchus xylophilus from Bursaphelenchus rnucronatus preliminarily iso-enzyme analysis of GOT was able to divide by rule and line two kinds of nematodes based on molecule biotechnology, ITS-RFLP method performed well in identifying two kinds of nematodes, and also in making judgments of terrain character of B. mucronatus which could be classified into Asian type or European type besides identified of B. xylophilus and B. mucronatus clearly, the RAPE） method was capable to describe the genetic relationship of interspecific and intraspecific in the evolution of each nematodes nicely.

关 键 词：松材线虫 鉴定方法 同工酶分析 ITS—RFLP分析 RAPD分析 

分 类 号：TQ78[化学工程]