油茶根腐病病原菌分子鉴定及其生物学特性研究  

Study on Biological Characteristics and Molecular Identification of Pathogens Causing Root Rot of Camellia oleifera

作　　者：李河[1] 周国英[1] 何末军[1] 

机构地区：[1]中南林业科技大学资源与环境学院,湖南长沙410004

出　　处：《西南林学院学报》2008年第5期45-48,56,共5页Journal of Southwest Forestry College

基　　金：国家"十一五"林业科技支撑计划项目(2006BAD08A1104)资助;国家林业局重点项目(2006-47)资助;湖南省教育厅科研项目(08C930)资助;中南林业科技大学青年科学研究基金项目(07003B)资助;中南林业科技大学研究生科技创新基金项目(2006sx10)资助

摘　　要：从油茶腐根中分离得到病原菌，通过对其形态特征的观察及该菌rDNA转录间隔区（ITS）序列的测定，与Genbank中经Blastn搜索获得的部分菌株构建分子系统发育树．基于形态特征和分子系统发育树，确定引起油茶根腐病的病原菌为镰刀属李瑟组层生镰刀菌（Fusarium proliferatum）．还对该菌的生物学特性进行了研究，发现其最适生长温度为25℃，最适pH值是6．0～7．0，最适碳源为可溶性淀粉，最适氮源为NH4Cl．Pathogens from rotten root of Camellia oleifera were isolated and identified. Molecular phylogenetic trees of some pathogen strains were expatiated through morphological characteristics observation, rDNA transcription, sequence determination of the internal transcribed spacer （ITS） and by Blastn search in the Genbank. Based on morphological characteristics and molecular phylogenetic trees, it was concluded that the pathogens to cause root rot of Camellia oleifera was Fusarium moniliform. The study on biological properties of the isolated pathogen showed that the optimal temperature for the pathogen was 25℃, the optimal pH range for its growth was 6 -7, the best carbon source was soluble starch and the best nitrogen source was NH4Cl.

关 键 词：油茶 根腐病 病原菌 层生镰刀菌 生物学特性 

分 类 号：S794.407[农业科学—林木遗传育种]