高寒牧草内生细菌分离培养条件的优化  

Optimization for isolation and culture conditions of endophytic bacteria in grasses in alpine area

作　　者：满百膺[1] 陈秀蓉[1] 李振东[1] 

机构地区：[1]甘肃农业大学草业学院草业生态系统教育部重点实验室中-美草地畜牧业可持续发展研究中心,甘肃兰州730070

出　　处：《草原与草坪》2008年第5期27-30,共4页Grassland and Turf

基　　金：甘肃农业大学草业学院草业科学国家级重点学科学术骨干科研项目暨草业生态系统教育部省部共建重点实验室项目(CY-GG-2006-01)资助

摘　　要：通过对高寒牧草内生细菌的分离培养方法研究，得出了优化的分离培养条件，即牧草不同组织器官用0．19／6SDS浸泡15min、3％NaClO浸泡3min、0．1％升汞浸泡10min、75％酒精1～2min处理后（各步间均用无菌水冲洗3～4次），研磨并稀释至10^-3，涂布于TSA培养基上，置28℃恒温条件下培养5—7d，可从高寒牧草组织中分离获得数量、种类较多的内生细菌。用此方法，已从5种牧草不同组织器官（根、茎、叶、花）中分离获得大小、形态、颜色各异的内生细菌315株。The optimized conditions for isolation and culture of endo alpine area in this study. The different tissues of grasses were immersed phytic bacteria isolated from grasses in in 0.1% of SDS for 15 min,3% of NaClO for 3 min,0.1% of mercuric chloride for 10 min, 75% of alcohol for 1 to 2 min. The endophytic bacteria were isolated after aseptic grinding and diluted to 10% and using TSA culture medium for 5 to 7 days under 28 ℃. 315 strains of endophytic bacteria with different size, shape and color in different tissues of five grasses were obtained.

关 键 词：高寒牧草 内生细菌 分离条件 优化 

分 类 号：Q93-335[生物学—微生物学] Q949.32