博莱霉素致大鼠肺纤维化形成过程中Thy-1表达变化与细胞因子生成之间关系的研究  

Correlation of Thy-1 on lung fibroblasts with cytokine in bleomycin-induced rat pulmonary fibrosis

作　　者：吕长俊[1] 张晓荣[1] 王晓芝[1] 高福泉[1] 郝东[1] 姚鲁肃[1] 

机构地区：[1]滨州医学院附属医院呼吸内科,256603

出　　处：《国际呼吸杂志》2008年第20期1224-1227,共4页International Journal of Respiration

摘　　要：目的测定博莱霉素致肺纤维化动物模型支气管肺泡灌洗液（bronchoalveo larlavage fluid，BALF）中白介素1（interleukin-1，IL-1）、肿瘤坏死因子α（tumor necrosis factor-α，TNF—α），研究其与肺成纤维细胞表面Thy-1表达阳性率之间的关系，探讨肺纤维化发病机制。方法于不同时间点采用免疫组织化学法测定Thy-1表达阳性率，采用胸腺细胞法测定BALF中IL-1的活性，采用结晶紫法测定TNF-α活性。结果肺纤维化形成过程中，成纤维细胞表面Thyd表达阳性率呈进行性下降，与IL-1、TNF-α活性之间无相关性。结论肺纤维化形成与Thy-1表达之间密切相关，Thy-1减少与IL-1、TNF-α无明显相关性，可能是一个综合因素作用的结果。Objective To measure expression of Thy-1 and content of cytokine in bronchoalveolar lavage fluid（BALF） in animal models of pulmonary fibrosis induced by bleomycin and observe relationship between expression of Tby-1 and cytokine and to approach pathogenesis of pulmonary fibrosis. Methods Expression of Thy-1 was observed by immunohistochemistry. Activity of interleukin-1 （IL-1） and tumor necrosis factor-α（TNF-α） in BALF were measured by thymocyte method and genitian violet respectively. Relationship between expression of Thy-1 and cytokine was analyzed. Results Expression of Thy-1 in fibroblasts decreased. Expression of Thy-1 about pulmonary fibrosis was uncorrelated with IL-1 and TNF-α. Conclusions Pulmonary fibrosis is closely correlated with Thy-1 expression. There are no obvious relevance between reduction of Thy-1 expression and IL-1 ,TNF-α. The disease may be a result of complex factors

关 键 词：Thy-1/CD90 肺纤维化 发病机制 白介素1 肿瘤坏死因子Α 

分 类 号：R563[医药卫生—呼吸系统]