三氧化二砷对类风湿性关节炎滑膜细胞凋亡影响的体外研究  

The in vitro study of the effects of arsenic trioxide on the synoviocytes apoptosis of rheumatoid arthritis.

作　　者：黄炜 李欣 张鸽[3] 李素香[4] 付桂茹[4] 袁静冉[4] 

机构地区：[1]河北省唐山市工人医院老年病科,063000 [2]河北省唐山市工人医院放射科,063000 [3]河北省唐山市工人医院风湿免疫科,063000 [4]华北煤炭医学院附属医院

出　　处：《中国综合临床》2008年第11期1163-1165,共3页Clinical Medicine of China

基　　金：河北省唐山市科学技术研究与发展计划（041346176）

摘　　要：目的类风湿性关节炎（Rheumatoid arthrithis，RA）滑膜细胞体外原代培养并传代至第三代，定量分析三氧化二砷（arsenic trioxide，As2O3）对滑膜细胞凋亡诱导作用。方法双酶消化法体外传代人关节滑膜细胞，采用3代滑膜细胞；应用MTT、流式细胞术对RA滑膜细胞凋亡进行分析。结果①MTT法显示不同浓度的As2O3作用48h后，能够抑制RA滑膜细胞增殖，且在10～80μmol／L浓度范围内其抑制作用呈剂量依赖性。②流式细胞仪检测发现不同浓度的As2O3作用48h后，RA滑膜细胞发生不同比率凋亡，并且在10～80μmol／L浓度范围内其作用呈剂量依赖性。结论不同浓度的As2O3作用48h后RA滑膜细胞均发生凋亡并且呈剂量依赖性。Objective To investigate arsenic trioxide （ As2O3 ）-mediated apoptosis of synovial cells in patients with rheumatoid arthritis （RA） through culturing the synoviocytes in vitro. Methods Primary synovial cells were cultured by means of two-enzymatic digestion and the third cells were adopted in this test. The cultured cells were defined by MTT and flow cytometry （ FCM ）. Results Certain concentration of As2O3 could inhibit the viability of synoviocytes at 48 h by means of MTT,which was dose-dependent. Certain concentration of As2O3 could induce the apoptosis of synoviocytes pro rata at 48h by means of FCM ,which was dose-dependent within range of 10-80 μmol/L concentration. Conclusion Certain concentration of As2O3 following 48 h effect could induce the apoptosis of synoviocytes of RA,which is dose-dependent.

关 键 词：关节炎 类风湿性 滑膜 细胞 凋亡 

分 类 号：R593.22[医药卫生—内科学]