CAC3基因转运肽序列和accD基因融合植物表达载体的构建  

Construction of Plant Expression Vector on Fusion Transit Peptide of CAC3 Gene and accD Gene

作　　者：张煜星[1,2] 崔燕[2] 武寒雪[2] 祝建波[2] 周鹏[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所国家重点实验室,海南海口571101 [2]石河子大学生命科学学院农业生物技术重点实验室,新疆石河子832003

出　　处：《华北农学报》2008年第5期26-29,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家“863”子项目(2007AA021401);兵团博士基金(2006JC07)

摘　　要：采用PCR方法分别从陆地棉和拟南芥基因组中扩增出Accase羧基转移酶-βCT亚基编码基因accD,Accase羧基转移酶-αCT亚基编码基因CAC3的定位于叶绿体的转运肽序列。分析结果与NCBI公布的氨基酸同源性分别为100%,99%。将CAC3基因转运肽序列与accD基因进行体外重组,构建了融合植物表达载体pBI-CAC3tp-accD,为下一步作物的遗传转化打下了基础。The acetyl-CoA earboxylase（ACCase） is the rate-limiting enzyme of fatty acids synthesis. The accD gene and transit peptide of CAC3 gene was amplified from C, ossyiuum hirsutum genome and Arabidopsis thaliana genome, DNA sequence analysis indicated that the cloned DNA fragmes have the highest homology compared with the NCBI published date,showing 100% and 99% .Plant expression vector of fusion transit peptide of CAC3 gene and accD gene was constructed. The vector of pBI-CAC3tp-accD will be usefull for further natural genetic transformation.

关 键 词：陆地棉 拟南芥 accD基因 CAC3基因 转运肽 

分 类 号：Q786[生物学—分子生物学]