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作 者:王娟[1] 桑丹[1] 轩淑欣[1] 李晓峰[1] 王彦华[1] 申书兴[1]
出 处:《华北农学报》2008年第5期89-92,共4页Acta Agriculturae Boreali-Sinica
基 金:河北省自然科学基金项目(C2005000222);河北农业大学科技将帅计划
摘 要:以大白菜-结球甘蓝异源三倍体(AAC,2n=3x=29)与二倍体大白菜(AA,2n=2x=20)回交一代BC1F1及其自交后代BC1F2为试验材料,经过细胞学观察和核型分析,于BC1F1中鉴定出了附加甘蓝2号染色体的大白菜-结球甘蓝单体异附加系,于BC1F2中鉴定出了附加甘蓝2号染色体的大白菜-结球甘蓝双体异附加系,并对其进行了减数分裂与植株形态学观察。该异附加系的获得为分析大白菜和甘蓝的亲缘关系,进一步获得大白菜和结球甘蓝易位系、代换系提供了基础材料。The backcross BC1F1 between allotripolid hybrids ( AAC, 2n = 3x = 29) and Chinese cabbage ( AA, 2n = 2x = 20)and self-fertilized progeny of it BC1F2 were used as materials in this study. A mosomic addition line named CO-2-1 (Chinese cabbage with chromosome 2 from cabbage)and a disomic addition line named CO-2-1D were obtained by cytological identification and karyotype analysis, and investigated the PMC meiosis behavior and the field character of the plants. The obtained alien addition lines would be used to analyzing the genetic relationship between Chinese cabbage and cabbage, and synthesizing their translocation lines and substitution lines.
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