活性炭对小白菜游离小孢子培养的影响  

Influence of Activated Charcoal on Isolated Microspore Culture in Chinese Cabbage

作　　者：蒋武生[1] 张晓伟[1] 姚秋菊[1] 原玉香[1] 耿建峰[1] 

机构地区：[1]河南省农业科学院园艺研究所,河南郑州450002

出　　处：《华北农学报》2008年第5期93-96,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家"863"计划资助项目(2001AA241125);河南省重大科技攻关项目(072101110400)

摘　　要：以24个小白菜品种为材料,通过在NLN培养基中添加不同浓度活性炭,研究其对游离小孢子培养的影响。结果表明：在NLN-13培养基中添加活性炭（0.5 g/L）,有利于小孢子胚的诱导和形成,供试材料（N4,N8）添加活性炭处理胚诱导率较未添加活性炭的处理分别提高19,66.5倍。采用添加活性炭（0.5 g/L）的NLN培养基对24份小白菜品种进行游离小孢子培养,有20份材料诱导出胚,培养成功率为83.3%。表明添加活性炭的培养基对小白菜游离小孢子培养有较好的效果。不同基因型间胚诱导率差别较大,每花蕾诱导胚数为0.4-86.7个。依据胚诱导率的高低可将其分为极易诱导、易诱导、难诱导和不能诱导出胚4类。B5＋6-BA 0.2 mg/L＋NAA 0.02 mg/L＋活性炭0.5 g/L＋3%蔗糖＋1%琼脂培养基有利于幼胚长成植株。Fluency of activated charcoal on isolated microspore culture in Chinese cabbage was studied. The results showed that NLN-1 3 liquid medium with activated charcoal （0.5 g/L）was obviously better in frequency of mierospore-derived embryos and frequency of regenerated plants. Frequency of microspore-derived embryos in N4 and N8 was increased 19 and 66.5 times. Isolated microspores from 24 varieties of Chinese cabbage were cultured in NLN-13 liquid medium with activated charcoal（0.5 g/L）, 20 varieties were produced mierospore-derived embryos. Embryo yield per bud （frequency microspore-derived embryos）was from 0.4 to 86.7.Mierospore-derived embryos on B5 medium with 6-BA 0.2 mg/L and NAA 0.02 mg/L and activated charcoal 0.5 g/L and 3% sucrose and 1% agar is good to regenerated plants.

关 键 词：小白菜 游离小孢子培养 小孢子胚 诱导率 活性炭 

分 类 号：S634.303.4[农业科学—蔬菜学]