SSR标记定位一个新的小麦白粉病抗性基因  

SSR Marker of a Novel Wheat Powdery Mildew Resistance Gene

作　　者：唐媛[1] 傅体华[1] 贾明娟[1] 

机构地区：[1]四川农业大学农学院,四川雅安625014

出　　处：《华北农学报》2008年第5期127-130,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金(30370770);四川省青年科技基金(05ZQ026-032)

摘　　要：来源于簇毛麦与普通小麦杂交后代的稳定小麦品系101-3含有1个新的抗白粉病显性基因,暂命名为PmX,用单体分析的方法已定位于染色体6B上。以感白粉病小麦品种中国春与101-3杂交后代F2为材料,用65对6B染色体上和9对6A染色体上小麦微卫星引物,进行连锁分析,发现小麦微卫星标记Xgwm570与基因的遗传距离为(9.72±2.40)cM,该结果表明,该基因位于小麦染色体6BL上,同时也为分子标记辅助育种上利用该基因提供了初步的选择标记。Wheat powdery mildew, caused by Erysiphe graminis f. sp. tritici is important fungal diseases of wheat in many regions in the world. A novel dominant powdery mildew resistance gene, temporarily named PmX, in wheat line 101- 3 derived from the progeny of the cross between common wheat and Dsypyrum villosum Candery L. was located on chromosome 6B by monosomic analyses. In the present study, SSR primers mapped in the chromosome 6B and 6A of common wheat were used for PCR amplification using the genomic DNA of resistant and susceptible bulks comprising of F2 individuals and their parents, 101-3 and Chinese Spring, to tag the powdery mildew resistance gene in line 101-3. No polymorphic products were observer in most primers. However, one primer Xgwm570, mapped on 6BL amplified polymorphic among resistant and susceptible bulks and their parents. The primer Xgwm570 was then used to amplify the F2 population from the cross 101-3/Chinese Spring. The result indicate that primer Xgwm 570 was linked to the resistance gene PmX with the genetic distance （9.72 ± 2.40）cM and suggesting the PmX was located in the chromosome 6BL of common wheat.The marker could be used in MAS of PmX in wheat breeding programs for powdery mildew resistance.

关 键 词：小麦 白粉病 抗性基因 SSR 

分 类 号：S435.121[农业科学—农业昆虫与害虫防治]