生物安全柜更换过滤器后防护性能的评价  

EVALUATION OF THE PROTECTION PERFORMANCE OF A BIOSAFETY CABINET AFTER REPLACING THE FILTER

作　　者：温占波[1] 赵建军[1] 王洁[1] 毕建军[1] 鹿建春[1] 李劲松[1] 

机构地区：[1]军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室,北京100071

出　　处：《解放军预防医学杂志》2008年第5期335-337,共3页Journal of Preventive Medicine of Chinese People's Liberation Army

基　　金：国家科技攻关计划项目课题(No.2004BA719A15)

摘　　要：目的测试与评价生物安全柜更换高效过滤器后对操作人员保护、产品保护和交叉污染的防护性能。方法制备枯草芽孢气溶胶发生液,按照标准中规定的测试方法,对安全柜的3种生物学评价指标,即人员保护、产品保护和交叉污染的防护性能进行测试,3种测试分别重复3次。结果3次测试中,人员保护每次6个液体撞击式采样器的枯草芽孢杆菌培养菌落数之和不超过10 cfu,2个固体采样器的培养菌落数之和不超过5 cfu。产品保护每次所有培养皿上的枯草芽孢杆菌落数不超过5 cfu。交叉污染每次测试中被检测侧壁36 cm外的所有培养皿上的枯草芽孢杆菌菌落数不超过2 cfu。结论该安全柜更换过滤器后,人员保护、产品保护和交叉污染的防护性能均良好,符合标准的要求。Objective To evaluate whether the protection performance of a biosafety cabinet after replacing the high efficiency particulate air filter is in accordance with the standard. Methods Prepare Subtilus bacillus aerosol. The protection of operation personnel, product, and crosscontamination were tested according to the standard. Each test was repeated three times. Results Personnel test showed that the sum of all liquid samplers were less than 10 cfu（colony formation unit） and that of all solid samplers less than 5 cfu. All product test showed less than 5 cfu. All cross contamination test were less than 2 cfu. Conclusion All of the three biological tests were in accordance with the standard requirement.

关 键 词：生物安全柜 防护性能 高效空气过滤器 评价 

分 类 号：R115[医药卫生—公共卫生与预防医学]