细粒棘球蚴延伸因子-1重组蛋白对小鼠的免疫保护性研究  

Immunoprotection of Recombinant Elongation Factor-1 Against Echinococcus Granulosus in Mice

作　　者：王健[1] 师志云[2] 赵巍[2] 

机构地区：[1]宁夏自治区人民医院,银川市750021 [2]宁夏医学院,银川市750004

出　　处：《中国药房》2008年第31期2408-2409,共2页China Pharmacy

基　　金：国家自然科学基金资助项目(30260105);宁夏自然科学基金资助项目(NZ0540)

摘　　要：目的:探讨细粒棘球蚴(Eg)延伸因子(Eg.EF-1)重组蛋白对小鼠的免疫保护性及其作为候选疫苗的潜在价值。方法:ICR小鼠随机分为免疫组和佐剂对照组,每隔2周皮下免疫1次,在第3次免疫后2周,用Eg原头节进行攻击感染,感染后20周剖杀小鼠,检获Eg包囊,计算免疫保护力,并用酶联免疫吸附法测定血清中免疫球蛋白(Ig)G及其亚型和IgE水平。结果:与佐剂对照组比较,免疫组小鼠的免疫保护力为85.6%,血清IgG、IgG1、IgG2a水平显著性升高(P<0.05),IgG2b水平轻微降低(P>0.05),IgG3和IgE水平无明显变化。结论:rEg.EF-1重组蛋白能诱导小鼠产生一定的免疫保护力,是潜在的疫苗候选抗原分子。OBJECTIVE： To investigate the protective immunity in mice immunized with rEg .EF- 1 and its potential as vaccine candidate. METHODS： ICR mice were subcutaneously immunized with rEg .EF- 1 for three times （with an interval of 2 weeks） and challenged with Eg 2 weeks after the completion the immunization, sacrificed at 20 weeks after infection with Eg. The rate of reduced hydatid cyst and the levels of IgE, IgG and IgG subclasses in sera were examined by enzyme linked immunosorbent assay, with adjuvant group as control. RESULTS： As compared with adjuvant group, the immunoprotection ability （rate of reduced hydatid cyst） in rEg .EF- 1 group was 85.6% ; The levels of IgG, IgG1 and igG2a in mice immunized with rEg .EF-1 significantly increased （P〈0.05）, while IgG 2b only had little decrease （P 〉 0.05）, and no significant difference was noted in IgG3 and IgE levels. CONCLUSIONS： The rEg.EF- 1 antigen could induce certain immunoprotection, suggesting the potential of rEg .EF- 1 as an vaccine candidate.

关 键 词：细粒棘球蚴 延伸因子 重组蛋白 免疫保护 

分 类 号：R977.6[医药卫生—药品] R965[医药卫生—药学]