太空搭载卤虫诱变效应的AFLP分子标记检测  

Genetic analysis on the effects of space-induced Artemia sinica with AFLP method

作　　者：刘波[1] 曾志南[1] 叶金聪[1] 林向阳[1] 郭明忠[1] 林光纪[1] 

机构地区：[1]福建省水产研究所,福建厦门361012

出　　处：《台湾海峡》2008年第4期417-421,共5页Journal of Oceanography In Taiwan Strait

基　　金：福建省海洋与渔业局资助项目

摘　　要：本文比较研究了经过太空诱变的卤虫（Artemia sinica）同地面对照组卤虫4对选择性扩增引物的AFLP指纹图谱，旨在探讨用AFLP技术进行诱变个体遗传差异检测的可行性．其遗传相似系数用Nei的计算方法进行计算，遗传距离则用Lynch的计算方法进行计算．实验共检测出384个位点，分子量在50～1500bp之间，其中绝大多数片段为太空诱变组同地面对照组所共有，多态位点73个，占19．0％．遗传指纹图谱显示：经过太空诱变的群体与地面对照组相比，从整个基因组角度来看，其遗传差异并不明显，但是太空诱变组有2个个体的基因确实产生了较为显著的变异．研究结果表明：太空诱变能够不定向地促进基因的变异，是水产新品种选育的途径之一；AFLP技术不仅是各水产动物种质鉴别的有效手段，用于各种诱变个体的遗传差异研究也是适宜和可信的．To explore the possibility of applying AFLP technology in analyzing genetic diversity of irradiated individuals,the AFLP fingerprinting of space-induced and control Anemia sinica were compared using 4 sets of selective primers for amplification. The genetic similarity coefficient was calculated by Nei＇ s matching coefficient method with designed computer program,and the correspondingly average genetic distance（D） was calculated by Lynch＇ s method to evaluate genetic structure. Amplification produced 384 distinctive and reproducible fingerprints that va- ried in length from 50 to 1 500bp in experiments, most of those were shared by space-induced and control group. 73 loci were polymorphic and the proportion was 19.0%. Genetic fingerprinting indicated low genetic variations between space-induced population and control population in genome, but some distinct genetic diversity could be found in two individuals of space-induced group. Thus the present study demonstrated that space induction which is one of approaches for aquaeulture breeding might cause variance in gene. AFLP teehnology is not only applicable in genetic studies within a species but also can be used in studies of phylogenesis and genetic diversity in aquatic animals.

关 键 词：卤虫 AFLP 太空诱变 

分 类 号：Q75[生物学—分子生物学]