鸡甲状旁腺素基因质粒对蛋鸡产蛋性能及钙磷代谢调控的研究  

Effects of pCEP4-PTH gene plasmid on production performance and calcium,phosphorus metabolism in layers

作　　者：章世元[1] 俞路[1] 王雅倩[1] 周联高[1] 严桂芹[1] 张莹[1] 林显华[1,2] 汪益峰[1,3] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]中国农业科学院北京畜牧兽医研究所,动物营养学国家重点实验室,北京100094 [3]江苏省农业科学院畜牧研究所,南京210014

出　　处：《安徽农业大学学报》2008年第4期517-523,共7页Journal of Anhui Agricultural University

基　　金：扬州大学博士科研启动基金项目(扬大人[2006]31号)资助

摘　　要：试验选用56周龄新扬州鸡产蛋鸡150只,随机分为3个处理,每个处理5个重复,各重复10只。研究经腿肌注射0、200和400μg鸡甲状旁腺素基因质粒(pCEP4-PTH)对蛋鸡产蛋量、蛋壳质量和钙磷代谢的影响。试验结果表明,pCEP4-PTH基因质粒可改善蛋鸡生产性能;200μg注射量可显著改善蛋壳强度(P<0.05),400μg注射量对蛋壳质量影响不显著;pCEP4-PTH基因质粒可在蛋鸡体内良好表达,甲状旁腺素(PTH)水平均显著升高(P<0.05),且注射400μg时达到极显著水平(P<0.01),注射400μg pCEP4-PTH基因质粒具有促进内源降钙素(CT)和雌激素(E2)分泌的作用(P<0.05),因而不利于其生物学效应的发挥,两试验组骨钙素(BGP)、碱性磷酸酶(AKP)与对照组差异不显著,血钙、血磷及钙、磷代谢率均有升高,表明pCEP4-PTH质粒不影响蛋鸡骨骼质量,且可促进其对钙、磷吸收代谢。pCEP4-PTH基因质粒可改善蛋鸡生产性能和蛋壳质量,促进钙、磷代谢吸收,且不影响骨骼质量。One hundred and fifty New Yangzhou layers of 56-week-old,were randomly divided into three treatments,each treatment had five repeats,and one repeat had 10 birds,to study the effects on production performance,eggshell quality,calcium and phosphorus appearance metabolic rates by injecting pCEP4-PTH gene plasmid into the crural muscle of layers with a dose of 0,200 and 400 μg,respectively.The results showed that pCEP4-PTH gene plasmid enhanced the production performance;Injecting dose of 200 μg obviously improved eggshell strength（P〈0.05）,injecting 400 μg had no effect on eggshell quality obviously;pCEP4-PTH gene plasmid might be expressed well in layers,the level of PTH elevated significantly（P〈0.05 or P〈0.01）,and in 400 μg dose it elevated extremely significantly（P〈0.01）;Injecting dose of 400 μg could promote the secretion of endogeny CT and E2（P〈0.05）,which was disadvantaged to display the biology effect,BGP and AKP in the two treatments were not significantly different from that of control group,serum calcium,phosphorus and calcium,phosphorus metabolism in the two treatments all increased,which indicated that pCEP4-PTH gene plasmid could improve the Absorption of calcium,phosphorus but would not be harmful to bone.pCEP4-PTH gene plasmid improved the production performance and eggshell quality,promoted calcium and phosphorus metabolism,but did not affect bone.

关 键 词：pCEP4-PTH基因质粒 蛋鸡 生产性能 蛋壳质量 激素 代谢率 

分 类 号：S831.5[农业科学—畜牧学]