Simultaneous electrochemical DNA hybridization assay for PAT and FMV 35S gene sequence using quantum dots as labels  

作　　者：Jiang Hua Zhong Peng Qin Wei Sun Kui Jiao 

机构地区：[1]College of Chemistry and Molecular Engineering, Key Laboratory of Eco-Chemical Engineering of Ministry of Education, Qingdao University of Science and Technology, Qingdao 266042, China

出　　处：《Chinese Chemical Letters》2008年第10期1244-1247,共4页中国化学快报（英文版）

基　　金：the National Natural Science Foundation of China(Nos.20405008,20635020).

摘　　要：An electrochemical method for the simultaneous detection of two different DNA sequences from PAT and FMV 35S gene sequence using CdS and PbS quantum dots （QDs） as labels was described. The QDs were readily functionalized with oligonucleotides as electrochemical DNA probes and selectively hybridized to the complementary sequences immobilized on the microplate. The QDs anchored on the hybrids were dissolved in the solution by the oxidation of HNO3 and further detected by a sensitive differential pulse anodic stripping voltammetric method （DPASV）. The DPASV signals of the oxidation of Cd^2＋ and Pb^2＋ ions present in the solution were different and reflected the identity of corresponding ssDNA targets sequences.An electrochemical method for the simultaneous detection of two different DNA sequences from PAT and FMV 35S gene sequence using CdS and PbS quantum dots （QDs） as labels was described. The QDs were readily functionalized with oligonucleotides as electrochemical DNA probes and selectively hybridized to the complementary sequences immobilized on the microplate. The QDs anchored on the hybrids were dissolved in the solution by the oxidation of HNO3 and further detected by a sensitive differential pulse anodic stripping voltammetric method （DPASV）. The DPASV signals of the oxidation of Cd^2＋ and Pb^2＋ ions present in the solution were different and reflected the identity of corresponding ssDNA targets sequences.

关 键 词：Electrochemical DNA assay Quantum dots Differential pulse anodic stripping voltammetry MICROPLATE Simultaneous detection 

分 类 号：O64[理学—物理化学]