分支杆菌热休克蛋白保守性B细胞表位经鼻粘膜的反复预免疫对随后攻击性免疫的强化作用  

The Strengthening Effect to Subsequence Aggressive Immunization of Pre-Immunization with Mycobacterium Tuberculosis' Conservative B Cell epitopes via Membrana Mucosa Nasi

作　　者：邓双玲[1] 贾绍辉[1] 熊祺琰[1] 张宇[1] 吴洁[1] 刘景晶[1] 

机构地区：[1]中国药科大学生命科学与技术学院微基因药学实验室,江苏南京210009

出　　处：《药物生物技术》2008年第5期325-330,共6页Pharmaceutical Biotechnology

基　　金：国家自然科学基金资助项目(No.30672464;No.30701023)

摘　　要：为了研究热休克蛋白65与动脉粥样硬化的关系,扩增出HSP65上与动脉粥样硬化密切相关的6个B细胞表位。将这6个表位分成两组,每组包含3个表位,其基因分别与CTB基因相融合,转化到大肠杆菌并表达蛋白。表达产物经过一系列的分离纯化步骤,并复性后经GM1-ELISA证明可在体外自组装成五聚体。用复性后的重组蛋白小剂量多次滴鼻免疫ICR小鼠,随后用大量的HSP65蛋白皮下免疫,ELISA检测小鼠血清中抗HSP65的抗体。初步药效学实验表明,重组蛋白预免疫ICR小鼠后,可以使免疫组较对照组对随后的大量HSP65刺激产生更加强烈的免疫反应。To investigate the relationship between HSP65 and atherosclerosis, 6 B cell epitopes which are closely correlated with atherosclerosis are cloned. These 6 epitopes were divided into 2 groups, 3 epitopes respectively. The genes of these epitopes were fusioned to CTB gene respectively and transformed into E. coli to express proteins. The expression product was purified by a series procedures and renaturation. GM1-ELISA manifests that the renaturation protein can assemble pentamers in vitro. Immune ICR mice with the renaturation protein via membrana mucosa nasi, and then immune them with high dose HSP65 by subcutaneous injection. The results of pharmacodynamics experiment indicate that mice immuned with the renaturation protein can response more strongly against HSP65 than the control.

关 键 词：HSP65/60 动脉粥样硬化 表位 免疫 粘膜 

分 类 号：Q514.3[生物学—生物化学]