腰痛宁胶囊HPLC指纹图谱研究  

HPLC fingerprint of Yaotongning Capsule

作　　者：艾立[1,2] 罗国安[2] 王义明[2] 

机构地区：[1]江西中医学院,江西南昌330004 [2]清华大学化学系,北京100084

出　　处：《中成药》2008年第10期1409-1412,共4页Chinese Traditional Patent Medicine

基　　金：国家十一五科技支撑计划(2006BAI08B04-01)

摘　　要：目的:建立腰痛宁胶囊(马钱子,乳香,没药等)指纹图谱的HPLC色谱分析条件,为腰痛宁胶囊内在质量评价积累数据,为优化及评价中药复方指纹图谱提供一种方法。方法:用均匀设计优化腰痛宁胶囊提取条件,利用高效液相色谱法(HPLC)建立了提取物的液相指纹图谱。考察了不同提取溶剂、提取条件对提取工艺的影响,最后用不同的相似度评价方法考察了成品之间的稳定性。结果:确立了最佳色谱条件,得到了腰痛宁胶囊的指纹图谱,标定了15个共有峰,包括士的宁,马钱子碱,甘草酸,蜕皮甾醇和麻黄碱,成品之间质量稳定可靠。结论:表明HPLC指纹图谱方法重现性好,稳定可靠,可用于腰痛宁胶囊指纹图谱研究。AIM： To establish the fingerprint analysis method of Yaotongning Capsule by HPLC-DAD. METHODS： This experiment used homogeneous design to optimize extract method, the analysis was performed on an Agilent C18（4.6 mm × 250 mm,5 μm. ）column with a acetonitrile-0.2 acetic acid, 0.2% triethylamine. Detection time was 60 min. The flow rate was 1.0 mL/min and the wavelength was at 254 nm. Effect of solvents time and method of extract on experiment was studied, then calculated fingerprint similarities to evaluate, the samples＇stability. RESULTS： 15 peaks including strychnine, brucine, glycyrrhizic acid, ecdy sterone and ephephedrine, were separated on HPLC fingerprint in Yaotongning Capsule. CONCLUSION： The method is reliable, accurate and can be used as a quality control for Yaotongning Capsule.

关 键 词：腰痛宁胶囊 均匀设计 相似度 指纹图谱 

分 类 号：R927.1[医药卫生—药学]