曼地亚红豆杉双黄酮结构表征和Taxol定量  

Structrue Identification of Biflavones and Determination of Taxol from Taxus Madia

作　　者：刘向前[1] 张晓丹[1] 朱育林[2] 申凡泳[3] 吴世旭 

机构地区：[1]中南大学制药工程系,湖南长沙410083 [2]华南师范大学化学与环境学院,广东广州510006 [3]庆熙大学汉方药理学教研室,韩国130-701 [4]韩国食品药品监督局,韩国122-704

出　　处：《中药材》2008年第10期1498-1501,共4页Journal of Chinese Medicinal Materials

摘　　要：目的：对曼地亚红豆杉黄酮类成分进行研究，对其不同药用部位紫杉醇含量进行测定。方法：采用反复硅胶柱层析进行分离纯化，根据理化性质、光谱特征鉴定其结构。采用HPLC法测定曼地亚红豆杉枝叶、主根、须根三种药用部位中紫杉醇的含量，色谱柱为C18ODS（150mm×4．6mm，5μm），流动相：甲醇-水（3：1），流速0．8ml／min，检测波长228nm。结果：从曼地亚红豆杉中分离得到三个双黄酮类化合物并进行结构鉴定为金松双黄酮（Ⅰ）、银杏黄素（Ⅱ）、7，7″，4＇-Tri—O—Methylamentoflavone（Ⅲ）。曼地亚红豆杉枝叶、主根、须根中紫杉醇含量依次为：0．0308、0．02191、0．01983mg／g。结论：化合物Ⅰ～Ⅲ为首次从该种植物中得到；曼地亚红豆杉中除树皮外的药用部位枝叶的含量最高，主根次之，须根含量最低。Objective ： To study the flavonoids from Taxus Madia and determine the content of Taxol from different parts of Taxus Madia. Methods：Various column chromatographic techniques were applied for isolation and purification. The structrues were elucidated by physico-chemical property and spectral evidence. The analysis of Taxol was carried out on C18 （ 150 mm ×4. 6 mm i. d. 5 μm） ,The mobile phase was MeOH-H2O （3：1 ） , The flow-rate was 0. 8 ml/min. The detection wave-length was 228nm. Results：Three bifiavonyl compounds were isolated and identified as Sciadopitysin（ Compound Ⅰ ）, Ginkgetin（ Compound Ⅱ ） ,7,7″,4＇-Tri-O-Methylamentoflavone（ Compound Ⅲ ）. The contents of Taxol from branches and leaves, main root, fibrous root was 0. 0308 mg/g,0. 02191 mg/g, 0. 01983 mg/g respectively. Conclusion：Compound Ⅰ - Ⅱ are obtained from Taxus Madia for the first time. The contents of Taxol is the highest in the part of branches and leaves ,and then is the main root ,the lowest is the fibrous root.

关 键 词：曼地亚红豆杉 金松双黄酮 银杏黄素 7 7” 4’-Tri-O-Methylamentoflavone 紫杉醇 含量测定 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]