超临界CO2萃取去除何首乌中的有害元素  

Harmful Elements Removal from Polygonum multiflorum by Supercritical CO_2 Extraction

作　　者：文震[1] 刘波[1] 郑宗坤[1] 游新奎[1] 蒲一涛[1] 党志[2] 

机构地区：[1]深圳大学化学与化工学院,广东深圳518060 [2]华南理工大学环境科学与工程学院,广东广州510640

出　　处：《中药材》2008年第10期1567-1571,共5页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(20606013);深圳大学科研启动基金(200816);广东省绿色化学产品技术重点实验室开放基金(GC200701)

摘　　要：目的:采用超临界CO2配合萃取技术脱除何首乌中有害元素Cu、Pb、As。方法:以二乙基二硫代氨基甲酸钠(NaDDC)作配合剂,乙醇为改性剂,考察了配合萃取方式、时间、压力、温度、配合剂用量等因素对何首乌中有害元素萃取的影响。结果:建立了有害元素脱除工艺为:萃取压力28MPa,萃取温度60℃,m(药材)∶m(NaDDC)=5∶1,m(药材)∶m(乙醇)=1∶1,静态萃取1 h,动态萃取2 h。在此条件下,何首乌中Cu、Pb、As的脱除率均达60%以上,而生理活性成分2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量保持不变。结论:本研究成果为寻找中药材中有害元素一种非破坏性的脱除方法提供了参考。Objective： To remove harmful elements as copper, lead and arsenic from Polygonum multiflorum, a Chinese traditional medicine, by supercritical carbon dioxide extraction. Methods： With sodium diethyldithiocarbamate （NaDDC） as a chelating agent and ethanol as a modifier, the effects of extraction style, time, pressure, temperature and amount of chelating agent on chelating extraction process were discussed. Results： The condition was optimized as following： 28MP as extraction pressure, 60℃ as extraction temperature, 1 h as static extraction time, 2h as dynamic extraction time, m（herb） ： m（NaDDC） = 5： 1, m（ herb）：m（ethanol） = 1：1. Under this condition, the extraction rate of Cu, Pb and As were up to 60% which achieved US FDA standard, while the physioloically active substance of 2,3,5,4＇-tetrahydroxy-silbene-2-O-β-D-glueoside was not extracted. Conclusion： The results show chelating extraction by supercritical CO2 can provide a non-destructive method to decrease the content of harmful elements from Chinese traditional medicines.

关 键 词：超临界 有害元素 配合 何首乌 

分 类 号：R284.2[医药卫生—中药学]