ABR和DPOAE检测研究NKCC1在不同基因型小鼠耳蜗听觉中的作用  

The Role of NKCC1 in Auditory Function of Transgenic Mice Detected by ABR and DPOAE

作　　者：褚汉启[1,2] 周良强[1,2] 王春芳[1,2] 黄孝文[1,2] 黄红彦[1,2] 崔永华[1,2] 孔维佳[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院耳鼻咽喉-头颈外科,武汉430030 [2]华中科技大学同济医学院附属耳鼻咽喉科学研究所,武汉430022

出　　处：《华中科技大学学报（医学版）》2008年第5期645-647,664,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No.30371526和No.30672307);湖北省自然科学基金资助项目(No.2002AB127)

摘　　要：目的应用听性脑干反应(ABR)和畸变产物耳声发射(DPOAE)技术检测不同基因型NKCC1小鼠的听觉功能,研究NKCC1在耳蜗听觉功能中的作用。方法利用ABR和DPOAE实验检测不同基因型NKCC1小鼠的听觉功能。结果NKCC1+/+野生型小鼠听力正常,ABR检测的短声(click)阈值为(23.13±3.78)dBSPL;NKCC1+/-杂合子小鼠听力低于NKCC1+/+野生型小鼠,其短声阈值为(38.49±12.29)dBSPL。NKCC1+/+和NKCC1+/-小鼠ABR的阈值均值在各个频率的差异有极显著性意义(均P<0.01);NKCC1-/-突变纯合子鼠的ABR的各个频率在100dB均无反应,呈现全聋。与NKCC1+/+小鼠比较,NKCC1-/-小鼠没有DPOAE的检出。结论耳蜗NKCC1在小鼠的听觉生理中有重要作用,NKCC1缺失或是功能受限均可影响耳蜗的听觉功能。Objective To detect the auditory function and investigate the role of NKCC1 in the cochlea of the mouse model with ABR and DPOAE technique. Methods Hearing threshold of ABR and DPOAE was measured on the NKCC1^-/- mice, NKCC1^-/- mice and NKCC1^＋/＋ mice respectively. Results The auditory function of NKCC1^＋/＋ mice was normal, and the mean value for ABR thresholds in response to click sound stimulus was （23.13 ± 3.78） dB SPL. The mean value for ABR thresholds in response to click sound stimulus in NKCC1^＋/- mice was （38.49± 12.29） dB SPL, significantly greater than in NKCC1^＋/＋ mice （P〈0.01）. NKCC1^-/- mice were completely deaf, and the ABR wave form was not observed for even 100 dB SPL sound stimuli. NKCC1^-/- mice did not produce DPOAEs as compared to wild-type mice. Conclusion The data indicated that NKCC1 plays a critical role in the auditory function of the mice. Loss or insufficiency of NKCC1 in cochlea can influence the hearing.

关 键 词：NKCC1 耳蜗 听力 听性脑干反应 畸变产物耳声发射 

分 类 号：R338.3[医药卫生—人体生理学]