硫酸乙酰肝素蛋白聚糖及肝素对巨噬细胞脂质蓄积的抑制作用  

The Inhibitory Effects of Heparin and Heparan Sulfate Proteoglycan on Accumulation of Lipid in Macrophages

作　　者：杨方[1] 赵培真[1] 蔡海江[1] 张英珊[1] 叶刚[1] 赵斌[1] 韩晓男[1] 

机构地区：[1]中国医学科学院中国协和医科大学心血管病研究所阜外心血管病医院

出　　处：《中国动脉硬化杂志》1997年第4期295-299,共5页Chinese Journal of Arteriosclerosis

基　　金：国家"八五"攻关课题!859150304

摘　　要：用苏丹Ⅳ染色观察巨噬细胞内脂质聚积的变化；通过酶－荧光法检测巨噬细胞内胆固醇含量并检测125Ⅰ标记氧化型低密度脂蛋白降解量来反映巨噬细胞清道夫受体的活性，以探讨硫酸乙酸肝素蛋白聚糖及肝素对巨噬细胞脂质蓄积的影响及机制。结果发现，氧化型低密度脂蛋白（100mg／L）组巨噬细胞的胞浆内可见较多苏丹Ⅳ着色红染颗粒，氧化型低密度脂蛋白（100mg／L）十硫酸乙酰肝素蛋白聚糖（15．5mg／L）组0及氧化型低密度脂蛋白（100mg／L）十肝素（200mg／L）组巨噬细胞内红色颗粒明显少于氧化型低密度脂蛋白组；氧化型低密度脂蛋白十硫酸乙酰肝素蛋白聚糖组及氧化型低密度脂蛋白十肝素组巨噬细胞内总胆固醇和胆固醇酯的含量明显低于氧化型低密度脂蛋白组（P＜0．05，P＜0．01）；硫酸乙酰肝素蛋白聚糖组及肝素组125Ⅰ标记的氧化型低密度脂蛋白降解量均低于对照组。结果提示，硫酸乙酰肝素蛋白聚糖和肝素可能是通过下调清道夫受体活性减少了巨噬细胞对氧化型低密度脂蛋白的摄取而抑制脂质在巨噬细胞内的聚集和泡沫细胞的形成。Aim To investigate the effect and mechanism of heparan sulfate proteoglycan and heparin on accumulation of lipid in macrophages.Methods Lipid accumulation in macrophages was observed with Sudan Ⅳ stain. Cholesterol content in macrophages was detected with enzyme fluorescence assay. The activity of scavenger receptor of macrophage was assessed by examing the degradation rate of 125 1 labeled oxidized low density lipoprotein (OLDL).Results More red particles stained with Sudan Ⅳ were seen in macrophages incubated with OLDL (100 mg/L). The red particles in macrophages incubated with OLDL (100 mg/L) plus heparan sulfate proteoglycan (HSPG, 15. 5 mg/L) or with OLDL (100 mg/L) plus heparin (HP, 200 mg/L) were far less than that with OLDL only. The total cholesterol and cholesteryl ester in macrophages incubated with OLDL plus HSPG or with OLDL plus HP were significantly lower than that with OLDL (P〈0.05: P〈0.01).The degradation rate of 125 I labeled OLDL of macrophages incubated with HSPG or with HP were lower than control.Conclusion HSPG and HP may inhibit lipid accumulation in macrophage and formation of foam cell possibly through down-regulating the activity of scavenger receptor of macrophage and decreasing the intake of OLDL.

关 键 词：动脉粥样硬化 HSPG 巨噬细胞 肝素 

分 类 号：R543.502[医药卫生—心血管疾病]